Our first PacBio Revio run with over 140Gb average HiFi yield! This was for a pool of 16 human WGS libraries across 8 SMRTcells, giving >20x coverage per sample. We're running these samples as part of the "Genome of Sweden" project 🧬🇸🇪

Our latest PacBio Revio run really delivered! Over 140Gb HiFi yield for the first 4 SMRT cells, and a new internal record at 150.1 Gb🎉 These are ~10 year old human biobank DNA samples, automated library prep. We've seen some variability during the spring but let's hope we can keep this up

I'm really excited about the prospect of reusable Revio SMRT cells! Dave Miller shared internal R&D results at the #PacBioPRISM meeting in Athens. Apparently, this has potential to increase the yield of one SMRT cell to 280Gb (dual use) or even to 500Gb (four uses)

Amazing talk by @lilimilani.bsky.social at #PacBioPRISM in Athens! Here, summarizing a comparison of 3 long read technologies and then giving a sneak peak of 2000 PacBio genomes from the Estonian biobank

The highest yields are achieved with libraries that have an average read length between 15-20kb and a tight size distribution. Our top runs in terms of total throughput are Kinnex bulk RNA libraries

Revio throughput varies a lot between different sample types. DNA from human blood usually works great, but other species and sample sources can be unpredictable... For cDNA and amplicons, the throughput is limited by the length of the SMRTbell libraries

Curious about what we’ve been running on PacBio Revio at @scilifelab-ngi.bsky.social?🧬🧪 Here are some end-of-2024 stats! We've processed many different sample types, but a major shift compared to the Sequel/Sequel II days is that we now are running a lot of human WGS