Alexander Ciattoni
@aciattoni.bsky.social
Plant Scientist @ipbhalle.bsky.social. Currently doing a PhD with @marischuster.bsky.social
I love the Bluesky community, but I regret Mastodon didn't become the path scientists followed, as it was the logical choice. We didn't learn from Twitter's mistakes and Bluesky risks becoming Twitter 2.0 in a few years. @fabmusacchio.bsky.social nicely summarised my concerns here: s.42l.fr/lJRVqxGY
Reflections on joining Bluesky: Opportunities and risks for the scientific community
Over the past two weeks, I have taken a closer look at Bluesky, exploring its features and dynamics through the lens of my own experience within the neuroscience community. So far, my impressions have...
s.42l.fr
August 27, 2025 at 8:23 PM
I love the Bluesky community, but I regret Mastodon didn't become the path scientists followed, as it was the logical choice. We didn't learn from Twitter's mistakes and Bluesky risks becoming Twitter 2.0 in a few years. @fabmusacchio.bsky.social nicely summarised my concerns here: s.42l.fr/lJRVqxGY
I can highly recommend the flowering inoculation protocol (doi.org/fmcxgb) as alternative to the dipping method. The easy version of it is: spin a 2mL o/n Agro culture and resuspend in 2mL 5%Sucrose + 0.02%Silwet L-77. Pipette ~5µL per bud cluster. For highest transformation rates repeat after 1week
The floral inoculating protocol: a simplified Arabidopsis thaliana transformation method modified from floral dipping
The floral dip protocol mediated by Agrobacterium tumefaciens is the most widely used transformation method for Arabidopsis thaliana. The “floral dip” …
doi.org
August 5, 2025 at 9:11 PM
I can highly recommend the flowering inoculation protocol (doi.org/fmcxgb) as alternative to the dipping method. The easy version of it is: spin a 2mL o/n Agro culture and resuspend in 2mL 5%Sucrose + 0.02%Silwet L-77. Pipette ~5µL per bud cluster. For highest transformation rates repeat after 1week
If presenters would use shortdoi.org this issue would be solved
shortDOI Service
shortdoi.org
July 22, 2025 at 8:49 AM
If presenters would use shortdoi.org this issue would be solved
I really hope to see this change! Yet I would recommend to use the shortdoi service ( www.shortdoi.org ) to generate a shorter ID, compared to PMID. It allows IDs for anything that has a DOI, ie preprints included
www.shortdoi.org
July 21, 2025 at 4:30 PM
I really hope to see this change! Yet I would recommend to use the shortdoi service ( www.shortdoi.org ) to generate a shorter ID, compared to PMID. It allows IDs for anything that has a DOI, ie preprints included