Valdir Barth
@valdirbarth.bsky.social
Rutgers alumni, former post doc at Boston Children's hospital. Now studying bacterial stress response in Brazil.
#microbiology #Tuberculosis
#microbiology #Tuberculosis
Reposted by Valdir Barth
Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
October 2, 2025 at 5:11 PM
Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
Reposted by Valdir Barth
from www.instagram.com/yeweijun98
Slime molds eat the microbes on decaying plants but not the microbes on decaying animals. Badhamia (Physarum) polycephala is an unusually adventurous eater who also enjoys mushrooms, oats, and probably a number of other things!
I wonder what's up with the peanut
Slime molds eat the microbes on decaying plants but not the microbes on decaying animals. Badhamia (Physarum) polycephala is an unusually adventurous eater who also enjoys mushrooms, oats, and probably a number of other things!
I wonder what's up with the peanut
July 5, 2025 at 4:40 PM
from www.instagram.com/yeweijun98
Slime molds eat the microbes on decaying plants but not the microbes on decaying animals. Badhamia (Physarum) polycephala is an unusually adventurous eater who also enjoys mushrooms, oats, and probably a number of other things!
I wonder what's up with the peanut
Slime molds eat the microbes on decaying plants but not the microbes on decaying animals. Badhamia (Physarum) polycephala is an unusually adventurous eater who also enjoys mushrooms, oats, and probably a number of other things!
I wonder what's up with the peanut
Reposted by Valdir Barth
Not only is the measles vaccine up to 99% effective at preventing measles infection, it can also reduce the risk of death from all causes by 30% up to 86% for up to 3 years (compared ro not being vaccinated) www.bbc.com/future/artic...
Measles: The race to understand 'immune amnesia'
Scientists have known for years that measles can alter the immune system – but the latest evidence suggests it's less of a mild tweaking, and more of a total reset.
www.bbc.com
April 27, 2025 at 8:11 PM
Not only is the measles vaccine up to 99% effective at preventing measles infection, it can also reduce the risk of death from all causes by 30% up to 86% for up to 3 years (compared ro not being vaccinated) www.bbc.com/future/artic...
Reposted by Valdir Barth
Journal editors are now receiving such letters from the US government. The entire scientific community must unite to support our journals in flatly resisting such illegal interventions.
April 17, 2025 at 9:28 PM
Journal editors are now receiving such letters from the US government. The entire scientific community must unite to support our journals in flatly resisting such illegal interventions.
Reposted by Valdir Barth
After 21 years at my dream job, I’m very sad to announce my early retirement from the National Institutes of Health. My life’s work has been to scientifically study how our food environment affects what we eat, and how what we eat affects our physiology.
April 16, 2025 at 9:00 PM
After 21 years at my dream job, I’m very sad to announce my early retirement from the National Institutes of Health. My life’s work has been to scientifically study how our food environment affects what we eat, and how what we eat affects our physiology.
Reposted by Valdir Barth
The NIH plans to resume posting notices of new solicitations for grant proposals. But there’s a catch: The HHS and DOGE will review each draft notice to ensure the research that will be funded aligns with the priorities of President Trump’s administration, Science has learned. scim.ag/3DJsafL
Trump officials will screen NIH funding opportunities
Staff at biomedical agency worry about political interference in setting priorities
scim.ag
March 27, 2025 at 12:47 PM
The NIH plans to resume posting notices of new solicitations for grant proposals. But there’s a catch: The HHS and DOGE will review each draft notice to ensure the research that will be funded aligns with the priorities of President Trump’s administration, Science has learned. scim.ag/3DJsafL
Reposted by Valdir Barth
A friend drove two hours today for her breast cancer chemotherapy only to learn it was canceled due to NIH cuts. Clinical trial ended midstream. No treatment, no restart, no plan, no information. Her bro-in-law's chemo was also canceled.
March 20, 2025 at 8:24 PM
A friend drove two hours today for her breast cancer chemotherapy only to learn it was canceled due to NIH cuts. Clinical trial ended midstream. No treatment, no restart, no plan, no information. Her bro-in-law's chemo was also canceled.
Reposted by Valdir Barth
Please repost: Postdoc position in antibiotic discovery in a large multidisciplinary project combining high-throughput approaches, microscopy, AI, and hit-to-lead optimization at @umontreal.ca and @mila-quebec.bsky.social. Apply to ML.Antibiotics@gmail.com. See
lnkd.in/dVVk4N3s. #microsky
lnkd.in/dVVk4N3s. #microsky
March 12, 2025 at 12:36 PM
Please repost: Postdoc position in antibiotic discovery in a large multidisciplinary project combining high-throughput approaches, microscopy, AI, and hit-to-lead optimization at @umontreal.ca and @mila-quebec.bsky.social. Apply to ML.Antibiotics@gmail.com. See
lnkd.in/dVVk4N3s. #microsky
lnkd.in/dVVk4N3s. #microsky
Reposted by Valdir Barth
Microbiome science needs more microbiologists- A new commentary authored by myself and @abaumler.bsky.social www.nature.com/articles/s41...
Microbiome science needs more microbiologists - Nature Microbiology
Microbiome science is a multi-disciplinary field, but classical microbiologists are needed to ensure advances are grounded in our understanding of basic microbiological concepts.
www.nature.com
January 23, 2025 at 5:14 PM
Microbiome science needs more microbiologists- A new commentary authored by myself and @abaumler.bsky.social www.nature.com/articles/s41...
Reposted by Valdir Barth
Ugggghhh cold chain shipping so expensive. I can only imagine what its like shipping cold internationally, especially to global south. A bioeconomy without local production is doomed to fail. Reagent access determines rate of bioresearch progress across the board. Raise the tide.
December 18, 2024 at 10:59 PM
Ugggghhh cold chain shipping so expensive. I can only imagine what its like shipping cold internationally, especially to global south. A bioeconomy without local production is doomed to fail. Reagent access determines rate of bioresearch progress across the board. Raise the tide.
Reposted by Valdir Barth
Today we report that an engineered skin bacterium, swabbed gently on the head of a mouse, can unleash a potent antibody response against a pathogen. Could lead to topical vaccines that are applied in a cream. @djenetbousbaine.bsky.social led the charge... @natureportfolio.bsky.social 1/55
December 11, 2024 at 4:29 PM
Today we report that an engineered skin bacterium, swabbed gently on the head of a mouse, can unleash a potent antibody response against a pathogen. Could lead to topical vaccines that are applied in a cream. @djenetbousbaine.bsky.social led the charge... @natureportfolio.bsky.social 1/55
Reposted by Valdir Barth
And now for the announcement:
While many of our plans for the award funds will take time to implement, one can start immediately.
To thank our users and encourage scientific debate, we’re introducing $1,000 rewards for selected PubPeer comments!
Yes students and postdocs you read that right: $1000
While many of our plans for the award funds will take time to implement, one can start immediately.
To thank our users and encourage scientific debate, we’re introducing $1,000 rewards for selected PubPeer comments!
Yes students and postdocs you read that right: $1000
November 25, 2024 at 8:45 AM
And now for the announcement:
While many of our plans for the award funds will take time to implement, one can start immediately.
To thank our users and encourage scientific debate, we’re introducing $1,000 rewards for selected PubPeer comments!
Yes students and postdocs you read that right: $1000
While many of our plans for the award funds will take time to implement, one can start immediately.
To thank our users and encourage scientific debate, we’re introducing $1,000 rewards for selected PubPeer comments!
Yes students and postdocs you read that right: $1000
Reposted by Valdir Barth
If you're interested in learning about the latest of fundamental mycobacteriology, sign up for #GRCMyco2025! Acceptances will start in September, check out the full program and please RT!
We're live with the program!
www.grc.org/biology-of-m...
www.grc.org/biology-of-m...
May 16, 2024 at 11:36 PM
If you're interested in learning about the latest of fundamental mycobacteriology, sign up for #GRCMyco2025! Acceptances will start in September, check out the full program and please RT!
Reposted by Valdir Barth
starter pack of academic imposters and it's just you
November 16, 2024 at 10:56 AM
starter pack of academic imposters and it's just you
Reposted by Valdir Barth
Please repost: Nov. 15 deadline! We are building a strong bacteriology community at Univ de Montréal, including recent hires Sven van Teeffelen, Frédérique Le Roux & myself. Moving to Montréal in 2018 is one of the best career decisions I made. Come join us! jobs.sciencecareers.org/job/664282/a...
Assistant or Associate Professor in Bacteriology - Montréal, Quebec (CA) job with University of Montreal | 664282
Université de Montréal (UdeM) is looking for an Assistant or Associate full-time professor in the field of bacteriology.
jobs.sciencecareers.org
November 8, 2024 at 1:23 PM
Please repost: Nov. 15 deadline! We are building a strong bacteriology community at Univ de Montréal, including recent hires Sven van Teeffelen, Frédérique Le Roux & myself. Moving to Montréal in 2018 is one of the best career decisions I made. Come join us! jobs.sciencecareers.org/job/664282/a...
The gut symbiont Akkermansia muciniphila can degrade mucin O-glycans including ABO epitopes - Which might be used as a way to transform A and B blood types into an O blood type turning everyone into an universal donor! That's cool 😎
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Akkermansia muciniphila exoglycosidases target extended blood group antigens to generate ABO-universal blood - Nature Microbiology
Discovery, biochemical and structural characterization of exoglycosidases from Akkermansia muciniphila reveals combinations that efficiently target extended A and B blood group antigens to produce ABO...
www.nature.com
April 30, 2024 at 7:17 PM
The gut symbiont Akkermansia muciniphila can degrade mucin O-glycans including ABO epitopes - Which might be used as a way to transform A and B blood types into an O blood type turning everyone into an universal donor! That's cool 😎
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Valdir Barth
Super useful and eminently readable demystification of writing a successful NIH grant application, written by NIH Program
Officers (POs) with LOTS of great guidance:
Officers (POs) with LOTS of great guidance:
Elements of successful NIH grant applications | Proceedings of the National Academy of Sciences
Is there a formula for a competitive NIH grant application? The Serenity Prayer may
provide one: "Grant me the serenity to accept the things I cann...
www.pnas.org
April 4, 2024 at 3:10 PM
Super useful and eminently readable demystification of writing a successful NIH grant application, written by NIH Program
Officers (POs) with LOTS of great guidance:
Officers (POs) with LOTS of great guidance:
Reposted by Valdir Barth
The Gates foundation says the preprint mandate is needed to accelerate the dissemination of research findings and because too many authors cannot afford publishing fees.
The journal Science turns this into: in a bold bid to avoid open-access fees, Gates foundation says grantees must post preprints.
The journal Science turns this into: in a bold bid to avoid open-access fees, Gates foundation says grantees must post preprints.
Posting #preprints is now a "bold bid to avoid open-access fees". 🙄
Anyway, great move by the Bill & Melinda Gates Foundation to mandate preprint posting for all grantees from 2025 onward! 👏
www.science.org/content/arti...
#ScientificPublishing
Anyway, great move by the Bill & Melinda Gates Foundation to mandate preprint posting for all grantees from 2025 onward! 👏
www.science.org/content/arti...
#ScientificPublishing
In a bold bid to avoid open-access fees, Gates foundation says grantees must post preprints
Foundation’s move leaves questions about how to fund peer review
www.science.org
April 3, 2024 at 3:04 PM
The Gates foundation says the preprint mandate is needed to accelerate the dissemination of research findings and because too many authors cannot afford publishing fees.
The journal Science turns this into: in a bold bid to avoid open-access fees, Gates foundation says grantees must post preprints.
The journal Science turns this into: in a bold bid to avoid open-access fees, Gates foundation says grantees must post preprints.
Reposted by Valdir Barth
T4 phage RNA is NAD-capped and alters the NAD-capepitranscriptome of Escherichia coli during infection through a phage-encoded decapping enzyme https://www.biorxiv.org/content/10.1101/2024.04.04.588121v1
T4 phage RNA is NAD-capped and alters the NAD-capepitranscriptome of Escherichia coli during infection through a phage-encoded decapping enzyme https://www.biorxiv.org/content/10.1101/2024.04.04.588121v1
Nicotinamide adenine dinucleotide (NAD) serves as a cap-like structure on cellular RNAs (NAD-RNAs) i
www.biorxiv.org
April 5, 2024 at 5:17 AM
T4 phage RNA is NAD-capped and alters the NAD-capepitranscriptome of Escherichia coli during infection through a phage-encoded decapping enzyme https://www.biorxiv.org/content/10.1101/2024.04.04.588121v1
Reposted by Valdir Barth
Non-mechanical disruption of Bacillus subtilis spores allows sensitive and deep characterization of the minimal proteome for resuming a cellular lifestyle. https://www.biorxiv.org/content/10.1101/2024.03.08.584050v1
Non-mechanical disruption of Bacillus subtilis spores allows sensitive and deep characterization of the minimal proteome for resuming a cellular lifestyle. https://www.biorxiv.org/content/10.1101/2024.03.08.584050v1
In response to extreme conditions, Bacillus subtilis generates highly resilient spores characterized
www.biorxiv.org
March 10, 2024 at 6:18 AM
Non-mechanical disruption of Bacillus subtilis spores allows sensitive and deep characterization of the minimal proteome for resuming a cellular lifestyle. https://www.biorxiv.org/content/10.1101/2024.03.08.584050v1
Reposted by Valdir Barth
This is very cool from Dave Grainger et al…
H-NS is a bacterial transposon capture protein www.biorxiv.org/content/10.1...
H-NS is a bacterial transposon capture protein www.biorxiv.org/content/10.1...
H-NS is a bacterial transposon capture protein
bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
www.biorxiv.org
February 17, 2024 at 8:54 AM
This is very cool from Dave Grainger et al…
H-NS is a bacterial transposon capture protein www.biorxiv.org/content/10.1...
H-NS is a bacterial transposon capture protein www.biorxiv.org/content/10.1...