www.protocols.io
@protocolsio.bsky.social
A secure platform for developing and sharing reproducible methods.
Reposted by www.protocols.io
👏 This month’s Network Spotlight goes to Teams Kordower, Liddle, & Rio!
Their open-access protocols ranked among the top 100 most viewed on @protocolsio.bsky.social in August 🎉
➡️ Mouse Perfusions (bit.ly/4qH6rIY)
➡️ Y-Maze (bit.ly/47aITTC)
➡️ Culture/transfection of HEK293T cells (bit.ly/4o4ytfR)
Their open-access protocols ranked among the top 100 most viewed on @protocolsio.bsky.social in August 🎉
➡️ Mouse Perfusions (bit.ly/4qH6rIY)
➡️ Y-Maze (bit.ly/47aITTC)
➡️ Culture/transfection of HEK293T cells (bit.ly/4o4ytfR)
October 31, 2025 at 3:05 PM
👏 This month’s Network Spotlight goes to Teams Kordower, Liddle, & Rio!
Their open-access protocols ranked among the top 100 most viewed on @protocolsio.bsky.social in August 🎉
➡️ Mouse Perfusions (bit.ly/4qH6rIY)
➡️ Y-Maze (bit.ly/47aITTC)
➡️ Culture/transfection of HEK293T cells (bit.ly/4o4ytfR)
Their open-access protocols ranked among the top 100 most viewed on @protocolsio.bsky.social in August 🎉
➡️ Mouse Perfusions (bit.ly/4qH6rIY)
➡️ Y-Maze (bit.ly/47aITTC)
➡️ Culture/transfection of HEK293T cells (bit.ly/4o4ytfR)
Reposted by www.protocols.io
Our new preprint covering the improvements we made to our respiratory metagenomic assay is out now:
www.medrxiv.org/content/10.1...
✅ Now implemented in 6 NHS hospitals
✅ Increased sensitivity
✅ Increased pathogen genome coverage
(1/3)
www.medrxiv.org/content/10.1...
✅ Now implemented in 6 NHS hospitals
✅ Increased sensitivity
✅ Increased pathogen genome coverage
(1/3)
Optimisation of whole cell human depletion provides increased sensitivity and microbial genome coverage from respiratory metagenomic assays
Metagenomics is being adopted worldwide as a laboratory developed test. We have previously reported a unified metagenomic method for the direct detection of microorganisms from respiratory samples, de...
www.medrxiv.org
October 20, 2025 at 10:35 AM
Our new preprint covering the improvements we made to our respiratory metagenomic assay is out now:
www.medrxiv.org/content/10.1...
✅ Now implemented in 6 NHS hospitals
✅ Increased sensitivity
✅ Increased pathogen genome coverage
(1/3)
www.medrxiv.org/content/10.1...
✅ Now implemented in 6 NHS hospitals
✅ Increased sensitivity
✅ Increased pathogen genome coverage
(1/3)
Reposted by www.protocols.io
Here’s a write-up that includes my lightning talk and reflections on why technicians must be recognised in the REF:
🔗 www.researchprofessionalnews.com/rr-news-uk-r...
Hope to work with others to get methods formally recognised as reportable research outputs in a category of their own.
🔗 www.researchprofessionalnews.com/rr-news-uk-r...
Hope to work with others to get methods formally recognised as reportable research outputs in a category of their own.
‘REF needs dedicated section for research methods’ - Research Professional News
Hidden REF Festival: Conference hears plea to fill gap in Research Excellence Framework
www.researchprofessionalnews.com
October 9, 2025 at 10:40 AM
Here’s a write-up that includes my lightning talk and reflections on why technicians must be recognised in the REF:
🔗 www.researchprofessionalnews.com/rr-news-uk-r...
Hope to work with others to get methods formally recognised as reportable research outputs in a category of their own.
🔗 www.researchprofessionalnews.com/rr-news-uk-r...
Hope to work with others to get methods formally recognised as reportable research outputs in a category of their own.
Explore our new featured protocols protocols.io/welcome
Bring structure to your research - protocols.io
A secure platform for developing and sharing reproducible methods.
protocols.io
October 6, 2025 at 6:31 PM
Explore our new featured protocols protocols.io/welcome
Reposted by www.protocols.io
I've been dreaming about this for a decade and it's now live!
"Global Collaboration Made Easier: Multilingual Protocols Now Available on protocols.io"
www.protocols.io/blog/global-...
"Global Collaboration Made Easier: Multilingual Protocols Now Available on protocols.io"
www.protocols.io/blog/global-...
Global Collaboration Made Easier: Multilingual Protocols Now Available on protocols.io
Research is a global endeavour, yet the expectation to communicate in English often creates barriers and an additional burden for non-native speakers. At the lab bench or in t...
www.protocols.io
October 2, 2025 at 6:45 PM
I've been dreaming about this for a decade and it's now live!
"Global Collaboration Made Easier: Multilingual Protocols Now Available on protocols.io"
www.protocols.io/blog/global-...
"Global Collaboration Made Easier: Multilingual Protocols Now Available on protocols.io"
www.protocols.io/blog/global-...
Reposted by www.protocols.io
We published a detailed protocol for our Plate-CUT&Tag method on @protocolsio.bsky.social to accompany our recent preprint (www.biorxiv.org/content/10.1...) give it a try! Feedback welcome! dx.doi.org/10.17504/protocols.io.n2bvjed5wgk5/v1
Plate CUT&Tag (Meers Lab version)
This protocol describes a method for carrying out CUT&Tag-Direct in 96 well microplates for high-throughput processing of samples by a single operator with only standard lab...
dx.doi.org
September 22, 2025 at 5:45 PM
We published a detailed protocol for our Plate-CUT&Tag method on @protocolsio.bsky.social to accompany our recent preprint (www.biorxiv.org/content/10.1...) give it a try! Feedback welcome! dx.doi.org/10.17504/protocols.io.n2bvjed5wgk5/v1
Reposted by www.protocols.io
Copy our protocols!
📝 Find many of our lab protocols and techniques on @protocolsio.bsky.social at www.protocols.io/search?q=%22...
🎥 Go deeper with recorded video tutorials of some of our most requested methods: alleninstitute.org/education/sc...
#OpenScienceWeek
📝 Find many of our lab protocols and techniques on @protocolsio.bsky.social at www.protocols.io/search?q=%22...
🎥 Go deeper with recorded video tutorials of some of our most requested methods: alleninstitute.org/education/sc...
#OpenScienceWeek
September 17, 2025 at 10:02 PM
Copy our protocols!
📝 Find many of our lab protocols and techniques on @protocolsio.bsky.social at www.protocols.io/search?q=%22...
🎥 Go deeper with recorded video tutorials of some of our most requested methods: alleninstitute.org/education/sc...
#OpenScienceWeek
📝 Find many of our lab protocols and techniques on @protocolsio.bsky.social at www.protocols.io/search?q=%22...
🎥 Go deeper with recorded video tutorials of some of our most requested methods: alleninstitute.org/education/sc...
#OpenScienceWeek
Reposted by www.protocols.io
We're hiring at protocols.io (@protocolsio.bsky.social) in NY or Jersey City - Associate Scientific Engagement Manager
See LinkedIn post for more info & feel free to message me: www.linkedin.com/posts/eganle...
See LinkedIn post for more info & feel free to message me: www.linkedin.com/posts/eganle...
August 14, 2025 at 10:09 AM
We're hiring at protocols.io (@protocolsio.bsky.social) in NY or Jersey City - Associate Scientific Engagement Manager
See LinkedIn post for more info & feel free to message me: www.linkedin.com/posts/eganle...
See LinkedIn post for more info & feel free to message me: www.linkedin.com/posts/eganle...
Reposted by www.protocols.io
Protocols @nmrfam.bsky.social Alex Paterson, Network for Advanced NMR Materials (NAN) Solid-State NMR Data Acquisition Protocols V.2 www.protocols.io/view/network... #NMRchat #NMR 🧲
Network for Advanced NMR Materials SSNMR Data Acquisition Protocols
The NAN Materials SSNMR Data Acquisition Protocols collection is part of the NAN Materials SSNMR Knowledgebase; a collection of resources, training and educational material, pul...
www.protocols.io
July 31, 2025 at 5:18 PM
Protocols @nmrfam.bsky.social Alex Paterson, Network for Advanced NMR Materials (NAN) Solid-State NMR Data Acquisition Protocols V.2 www.protocols.io/view/network... #NMRchat #NMR 🧲
Reposted by www.protocols.io
Author here to answer any questions. The full protocol is online at protocols.io and all codebooks on github
Bring structure to your research - protocols.io
A secure platform for developing and sharing reproducible methods.
protocols.io
July 29, 2025 at 7:48 PM
Author here to answer any questions. The full protocol is online at protocols.io and all codebooks on github
Reposted by www.protocols.io
Load gels? Use #zebrafish 🐟? Want to do more sustainable research w/out compromising science? We-G Gestri, K Turner, G Powell @stevewilsonlab.bsky.social-made these protocols. Yes, we use these in our @science.org+@nature.com papers.
www.protocols.io/view/re-usin...
www.protocols.io/view/washing...
www.protocols.io/view/re-usin...
www.protocols.io/view/washing...
Re-using pipette tips for gel loading
Gel loading tips can be reused for most applications; there’s REALLY no need to throw them away after a single use. In
our labs, we routinely reuse tips when loading gels, onl...
www.protocols.io
July 22, 2025 at 4:02 PM
Load gels? Use #zebrafish 🐟? Want to do more sustainable research w/out compromising science? We-G Gestri, K Turner, G Powell @stevewilsonlab.bsky.social-made these protocols. Yes, we use these in our @science.org+@nature.com papers.
www.protocols.io/view/re-usin...
www.protocols.io/view/washing...
www.protocols.io/view/re-usin...
www.protocols.io/view/washing...
Reposted by www.protocols.io
Comparative Analysis of Phage Profiles in Environmental Samples: Metagenomic Sequencing Versus Culture-Based Isolation www.protocols.io/view/compara...
Comparative Analysis of Phage Profiles in Environmental Samples: Metagenomic Sequencing Versus Culture-Bas...
Culture-based isolation of bacteriophages from wastewater relies on a limited set of laboratory‐cultivable host strains, causing it to recover only a small fraction of the tru...
www.protocols.io
July 22, 2025 at 6:26 PM
Comparative Analysis of Phage Profiles in Environmental Samples: Metagenomic Sequencing Versus Culture-Based Isolation www.protocols.io/view/compara...
Reposted by www.protocols.io
Happy to answer any and all questions about this work. We'd love to see more people try it.
Full protocol: www.protocols.io/view/craftse...
Full analysis: github.com/immunogenomi...
Full protocol: www.protocols.io/view/craftse...
Full analysis: github.com/immunogenomi...
CRAFTseq
Genetic studies have identified thousands of individual disease-associated non-coding alleles, but identification of the causal alleles and their functions remain critical bottl...
www.protocols.io
July 23, 2025 at 3:22 PM
Happy to answer any and all questions about this work. We'd love to see more people try it.
Full protocol: www.protocols.io/view/craftse...
Full analysis: github.com/immunogenomi...
Full protocol: www.protocols.io/view/craftse...
Full analysis: github.com/immunogenomi...
Reposted by www.protocols.io
I am very excited to share our publication, out now in Nature, detailing our developed CRAFTseq methodology for capturing and analysing single cell genomic DNA amplicons alongside the transcriptome and cell surface protein expression.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Precisely defining disease variant effects in CRISPR-edited single cells - Nature
A plate-based assay called CRAFTseq has been developed that uses ‘multi-omic’ single-cell RNA sequencing and direct genotyping of CRISPR edits to test the functional effects of genetic variants o...
www.nature.com
July 23, 2025 at 3:21 PM
I am very excited to share our publication, out now in Nature, detailing our developed CRAFTseq methodology for capturing and analysing single cell genomic DNA amplicons alongside the transcriptome and cell surface protein expression.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by www.protocols.io
The easiest publishing process ever! After testing & retesting this protocol adapted from a large-volume industry standard method, I finally clicked “Publish” over at @protocolsio.bsky.social today. Can recommend; would publish again!! 🌾 🍞 🧪
dx.doi.org/10.17504/pro...
dx.doi.org/10.17504/pro...
Microplate Beta Glucan Assay
This assay measures the mixed linkage beta glucan content of cereal grain flour through specific, enzyme-catalysed, step-wise hydrolysis of the polysaccharide to glucose and qua...
dx.doi.org
July 4, 2025 at 10:56 AM
The easiest publishing process ever! After testing & retesting this protocol adapted from a large-volume industry standard method, I finally clicked “Publish” over at @protocolsio.bsky.social today. Can recommend; would publish again!! 🌾 🍞 🧪
dx.doi.org/10.17504/pro...
dx.doi.org/10.17504/pro...
Reposted by www.protocols.io
🚨🚨 We just published a protocol for non-lethally measuring morphology in 3D in small aquatic organisms!! 🚨🚨 www.protocols.io/view/non-let...
The work was led by undergrad Daniela Granados 🎉🎉 and our collaborators include Najva Akbari, and Lauren O'Connell!
The work was led by undergrad Daniela Granados 🎉🎉 and our collaborators include Najva Akbari, and Lauren O'Connell!
Non-lethal 3-D imaging techniques for estimating tadpole morphology
The majority of protocols for imaging biological specimens are lethal and this raises both ethical and scientific concerns. One major limitation of most imaging protocols is tha...
www.protocols.io
June 26, 2025 at 1:45 PM
🚨🚨 We just published a protocol for non-lethally measuring morphology in 3D in small aquatic organisms!! 🚨🚨 www.protocols.io/view/non-let...
The work was led by undergrad Daniela Granados 🎉🎉 and our collaborators include Najva Akbari, and Lauren O'Connell!
The work was led by undergrad Daniela Granados 🎉🎉 and our collaborators include Najva Akbari, and Lauren O'Connell!
Reposted by www.protocols.io
Last month we published our protocol for detection of beet yellows virus. Here it is!
RT-LAMP for BYV detection www.protocols.io/view/rt-lamp...
RT-LAMP for BYV detection www.protocols.io/view/rt-lamp...
RT-LAMP for BYV detection
This protocol describes the process of detecting beet yellows virus (BYV) in plant material utilising a quick and easy colorimetric assay. The starting point is total RNA extrac...
www.protocols.io
June 11, 2025 at 9:56 AM
Last month we published our protocol for detection of beet yellows virus. Here it is!
RT-LAMP for BYV detection www.protocols.io/view/rt-lamp...
RT-LAMP for BYV detection www.protocols.io/view/rt-lamp...
Reposted by www.protocols.io
qPCR diagnosis of #diphtheria: you may be interested by our procedure, which is now widely used across European reference laboratories: www.protocols.io/view/identif... @EURLdiphtheriaPertussis thanks @chcrestani.bsky.social for releasing on protocols.io
Identification of Corynebacteria of the diphtheriae Species Complex and detection of the diphtheria toxin g...
This protocol describes a quadruplex real-time PCR (Dip4plex), performed with the Qiagen QuantiNova™ Multiplex PCR Kit, for simultaneous identification of Corynebacteria of th...
www.protocols.io
June 6, 2025 at 11:50 AM
qPCR diagnosis of #diphtheria: you may be interested by our procedure, which is now widely used across European reference laboratories: www.protocols.io/view/identif... @EURLdiphtheriaPertussis thanks @chcrestani.bsky.social for releasing on protocols.io
Reposted by www.protocols.io
Happy to be in Brno for the #CTLS2025 conference from the Core Technologies for Life Sciences society. I'll be talking about protocols.io tomorrow morning in the Core Facilities and Open Science session
June 10, 2025 at 9:42 AM
Happy to be in Brno for the #CTLS2025 conference from the Core Technologies for Life Sciences society. I'll be talking about protocols.io tomorrow morning in the Core Facilities and Open Science session
Spotlight interview with Maria Diehl regarding her PLOS ONE Lab Protocol paper: "Modifying the platform-mediated avoidance task: A new protocol to study active avoidance within a social context in rats." @realdiehllab.bsky.social
Protocol Spotlight with Maria Diehl
YouTube video by protocolsio
youtu.be
June 6, 2025 at 7:30 PM
Spotlight interview with Maria Diehl regarding her PLOS ONE Lab Protocol paper: "Modifying the platform-mediated avoidance task: A new protocol to study active avoidance within a social context in rats." @realdiehllab.bsky.social
Reposted by www.protocols.io
For scientific writers: Three things your Methods section shouldn't be - but three things Methods sections often end up being. You can avoid these mistakes! scientistseessquirre...
What your Methods section isn’t
If you’re a scientist like me, you read a lot of papers – and a lot of submitted manuscripts, drafts, and other larval stages of papers.* This is extremely helpful when it’s time to write your own …
scientistseessquirrel.wordpress.com
June 3, 2025 at 11:36 AM
For scientific writers: Three things your Methods section shouldn't be - but three things Methods sections often end up being. You can avoid these mistakes! scientistseessquirre...
Reposted by www.protocols.io
A fantastic start to #OSFest25 this morning, looking at publishing methods and protocols with @protocolsio.bsky.social. With three different sessions tomorrow and more over the week ahead, it’s not too late to have a look at the festival schedule and register your place: buff.ly/jZsSd6U
June 2, 2025 at 12:02 PM
A fantastic start to #OSFest25 this morning, looking at publishing methods and protocols with @protocolsio.bsky.social. With three different sessions tomorrow and more over the week ahead, it’s not too late to have a look at the festival schedule and register your place: buff.ly/jZsSd6U
Reposted by www.protocols.io
This month, we are recognizing the Assessment of Motor and Non-Motor PD Symptoms Working Group as our Network Spotlight! They shared protocols to support more reproducible rodent behavioral assays in #ParkinsonsDisease research 🐁 🧠
🔗 Check them out: www.protocols.io/view/behavio...
🔗 Check them out: www.protocols.io/view/behavio...
May 30, 2025 at 3:46 PM
This month, we are recognizing the Assessment of Motor and Non-Motor PD Symptoms Working Group as our Network Spotlight! They shared protocols to support more reproducible rodent behavioral assays in #ParkinsonsDisease research 🐁 🧠
🔗 Check them out: www.protocols.io/view/behavio...
🔗 Check them out: www.protocols.io/view/behavio...
Reposted by www.protocols.io
Today. bsky.app/profile/ltey...
Tomorrow: webinar with @ruthtimme.bsky.social, "Improving Global Pathogen Surveillance: Leveraging Open Science Through Standardized Protocols". go.springernature.com/improving-gl...
May 29, 2025 at 2:29 PM
Today. bsky.app/profile/ltey...