Plant Disease
LightNews LightNews
banner
plantdiseasej.bsky.social
Plant Disease
@plantdiseasej.bsky.social
180 followers 2 following 59 posts
The leading international journal for rapid reporting of research on new, emerging, and established plant diseases. Published by The American Phytopathological Society.
Posts Replies Media Videos
plantdiseasej.bsky.social
Special Report: “A Rapid On-Site Visualization Detection System for Fusarium oxysporum f. sp. cubense Tropical Race 4 Utilizing RPA-CRISPR/Cas12a,” by Lili Zhu et al. Learn more: https://doi.org/10.1094/PDIS-11-24-2512-SR
Fig. 8. RPA-CRISPR/Cas12a system-based process to detect Foc TR4.
December 8, 2025 at 4:52 PM
plantdiseasej.bsky.social
Sai Wang et al. designed a specific primer pair (XfOG4-F/XfOG4-R) through comparative genomic analysis of 660 genomes from the genus #Xanthomonas for the rapid detection of Xanthomonas fragariae in strawberry. Learn more: https://doi.org/10.1094/PDIS-11-24-2299-RE
Fig. 6. PCR detection of Xanthomonas fragariae in strawberry samples. A, PCR detection of X. fragariae in artificially inoculated strawberry leaves. M: 50 bp DNA ladder; L0: positive control (X. fragariae W2L gDNA); L1 to L5: 1 d, 2 d, 3 d, 4 d, and 5 d; L6: normal leaf; L7: ddH2O. B, PCR detection of X. fragariae in naturally infected strawberry plants from farms. M: 50 bp DNA ladder; L1 to L4: infected leaves from four strawberry plants; L5: positive control (X. fragariae W2L gDNA); L6: ddH2O. Genomic DNA from the infected leaves (L1 to L6 in A and L1 to L4 in B) was extracted using the TIANGEN Plant Genomic DNA Extraction Kit according to the manufacturer’s instructions.
December 5, 2025 at 8:10 PM
plantdiseasej.bsky.social
Special Report: “Development of a Triplex Quantitative PCR System for the Detection of Parastagonospora nodorum, Zymoseptoria tritici, and Pyrenophora tritici-repentis from Wheat,” by Heting Fu et al. Read the article to learn more: https://doi.org/10.1094/PDIS-01-25-0003-SR
Fig. 1. Locations in Alberta municipal districts of 145 wheat leaf samples that were collected from different fields for leaf spot detection. In each municipal district, the number of samples was indicated. Details regarding the samples can be found in Supplementary Table S2.
December 4, 2025 at 9:04 PM
plantdiseasej.bsky.social
Ying He et al. identified a biocontrol agent—Bacillus velezensis XJ20—and assessed the antifungal efficacy against Stagonosporopsis caricae, a fungal isolate obtained from rotten pumpkin fruits. Learn more: https://doi.org/10.1094/PDIS-11-24-2394-RE
Fig. 1. Morphological characteristics of isolates causing pumpkin fruit rot. A, Symptoms of pumpkin fruit rot in storage. B and C, Colonies of isolates. D and E, Conidiophores and conidia. F, Symptoms of pumpkin fruit rot inoculated with isolates.
December 3, 2025 at 4:47 PM
plantdiseasej.bsky.social
Charcoal rot of soybean is a major disease of economic significance worldwide. Alemu Mengistu et al. evaluated new methods to identify resistant and susceptible genotypes when inoculated with the causal fungus in nonfield environments. https://doi.org/10.1094/PDIS-10-24-2230-RE
Fig. 1. Experiment 1. The posterior probability of correctly classifying moderately resistant (MR; dark bars) and susceptible (S; light bars) soybean genotypes for each Macrophomina phaseolina isolate given the model fit to the data from experiment 1, the growth chamber study. The MR genotype is DT97-4290, and the S genotype is LS98-0358.
December 2, 2025 at 4:54 PM
plantdiseasej.bsky.social
Special Report: “Systematic Surveillance of Fusarium Head Blight in the Southern Region of the Republic of Korea,” by Hosung Jeon et al. Learn more: https://doi.org/10.1094/PDIS-02-25-0282-SR
Fig. 2. Occurrences of Fusarium head blight (FHB) outbreak in the Republic of Korea from 2021 to 2024. A, Spatiotemporal dynamics of FHB outbreaks in the Republic of Korea during 2021 to 2024. On the map, the severity of FHB outbreaks (percentage of infected spikes) is depicted by the size of the circles, while deoxynivalenol/nivalenol concentrations (μg/kg) are represented by the intensity of the red color. Box plots denote the percentage of infected spikes in B, wheat and C, barley from 2021 to 2024. Box plots indicating concentrations (μg/kg) of deoxynivalenol are shown in D, wheat and E, barley from 2021 to 2024. Statistical significances are denoted by P values.
December 1, 2025 at 4:48 PM
plantdiseasej.bsky.social
In Chile, data on fungicide efficacy against apple twig and branch pathogens are limited. Adrián Valdez-Tenezaca et al. tested fungicides against #Botryosphaeria canker and dieback in vitro, in greenhouses, and in the field. Learn more: https://doi.org/10.1094/PDIS-11-24-2427-RE 🍎
Efficacy of Pruning-Wound Protectant Fungicides for the Management of Botryosphaeria Canker and Dieback of Apple Trees in Maule Region, Chile | Plant Disease
Botryosphaeria canker and dieback is an important fungal disease caused by Botryosphaeriaceae spp. that affects the productivity of apple orchards in Chile and worldwide. In the field, studies on the management of this disease are focused on the protect...
doi.org
November 28, 2025 at 8:07 PM
plantdiseasej.bsky.social
Jonathan Phillips et al. show that trace amounts of ‘Candidatus Liberibacter asiaticus’ (CLas) can go undetected in qPCR assays when the internal standard is too abundant or too dissimilar to CLas. Learn more: https://doi.org/10.1094/PDIS-12-24-2714-RE
Fig. 3. Impact of citrus leaf blotch virus (CLBV) amplicon concentration on internal standard–CLBV (IS-CLBV) detection. The top schematic illustrates the IS-CLBV internal standard, consisting of a short sequence from CLBV (green font), flanked by the forward and reverse primers along with three to four downstream nucleotides of the CLas target sequence (blue and black lines, respectively). The plot displays cycle threshold (Ct) values from quantitative PCR (qPCR) reactions containing approximately 21 molecules of IS-CLBV standard, varying concentrations of CLBV amplicon, Huanglongbing (HLB) primers, and a hydrolysis probe that binds to both IS-CLBV and CLBV amplicon. Each CLBV amplicon concentration was tested in seven qPCR replicates (n = 7). The dashed green line represents the fitted regression, with the shaded green area indicating the 95% confidence interval.
November 26, 2025 at 5:08 PM
plantdiseasej.bsky.social
Special Report: “Multiplexed Real-Time and Digital PCR Tools to Differentiate Clades of Plasmopara viticola Causing Downy Mildew in Grapes,” by Lexi Heger et al. Read the article to learn more: https://doi.org/10.1094/PDIS-01-25-0173-SR
November 25, 2025 at 5:05 PM
plantdiseasej.bsky.social
Jun Wang et al. developed species-specific primers to facilitate the rapid identification of #Verticillium species. Learn more: https://doi.org/10.1094/PDIS-10-24-2227-RE
Fig. 5. Evaluation of the species specificity of primer sets for amplification of genomic DNA template of two Verticillium species from infected plant samples. The ladder sizes are indicated by the “>” symbol. A, Detection of V. dahliae from inoculated cotton. The primer pairs in lanes 1 to 10 were Vd-F/R, Vl-F/R, Vt-F/R, Vi-F/R, Vk-F/R, Vn-F/R, Valfa-F/R, Valbo-F/R, Vnu-F/R, and Vz-F/R, respectively. B, Detection of V. isaacii from inoculated spinach. The primer pairs in lanes 1 to 10 were Vi-F/R, Vl-F/R, Vt-F/R, Vk-F/R, Vn-F/R, Valfa-F/R, Vd-F/R, Valbo-F/R, Vnu-F/R, and Vz-F/R, respectively.
November 24, 2025 at 5:08 PM
plantdiseasej.bsky.social
Shani Friedman et al. explored the potential of quantitative whole-plant physiological analysis over traditional visual estimates for understanding and detecting plant diseases. Learn more: https://doi.org/10.1094/PDIS-07-24-1523-RE
Fig. 1. PlantArray system description.
November 21, 2025 at 8:10 PM
plantdiseasej.bsky.social
Special Report: “Rapid Detection of Frogeye Leaf Spot Pathogen in Seeds by LAMP Assays to Protect Soybean Production,” by Xiaoshan Tan et al. Read the article to learn more: https://doi.org/10.1094/PDIS-10-24-2156-SR
Fig. 1. Frogeye leaf spot (FLS) disease on leaves of soybean in the field and isolation of Cercospora sojina strain. A, Typical FLS disease lesions with gray centers and reddish-brown margins on soybean leaves. B, Pure culture of the C. sojina strain from a single spore on PDA medium. C, Reddish-brown spots were induced by spore suspension of C. sojina strain using needleless syringe inoculation.
November 20, 2025 at 9:22 PM
plantdiseasej.bsky.social
To facilitate research on tomato brown rugose fruit virus (ToBRFV), Mareike J. Rohde, Annette Niehl, and Heiko Ziebell constructed a full-length clone of the ToBRFV strain PV1241 (designated pJL89-ToBRFV) using Gibson assembly: https://doi.org/10.1094/PDIS-08-24-1665-RE
Fig. 4. Symptoms induced by pJL89-ToBRFV on various host plants. Plants were inoculated with phosphate buffer (Mock), leaf sap from Nicotiana tabacum ‘Samsun nn’ plants previously infected with pJL89-ToBRFV 3 weeks before (ToBRFV rub inoculation) or infiltrated with Agrobacteria GV3101 carrying pJL89-ToBRFV (tomato brown rugose fruit virus [ToBRFV] infiltration). Pictures are marked with a red frame when ToBRFV was detected in systemic leaves by quantitative reverse transcriptase PCR. The images were edited with Inkscape version 1.2 to crop and adjust brightness (Inkscape Project 2022).
November 19, 2025 at 5:07 PM
plantdiseasej.bsky.social
Feature article! 🚨 Daniela E. Cardenas et al. explore the latest advances in detection, control, and cultural practices that can be integrated or stand alone to help thwart rot caused by fungal and bacterial postharvest pathogens of fruit: https://doi.org/10.1094/PDIS-10-24-2263-FE
Fig. 7. Postharvest symptoms of Colletotrichum spp. causing ripe rot in Chardonnay grapes. Colletotrichum spp. initially cause small, dark brown to black spots on the grape surface. As the disease progresses, lesions expand, and orange to salmon-colored spore masses develop. Infected grapes soften, sink in, and may eventually shrivel and mummify with dark brown color. (Photographs courtesy of Charlotte Oliver).
November 18, 2025 at 4:52 PM
plantdiseasej.bsky.social
Editor’s Pick: “Potential for Elite Veneer Poplar on Marginal Phytophthora spp.–Infected Christmas Tree Lands,” by William Henry Kohlway et al.: https://doi.org/10.1094/PDIS-07-24-1558-RE 🌲
Fig. 1. Phytophthora root rot pathogenicity trials.
November 17, 2025 at 5:15 PM
plantdiseasej.bsky.social
Oadi Matny, Michael D. Miller, and Brian Joel Steffenson demonstrate that Hordeum jubatum can be infected by isolates of important barley pathogens but typically not with the same degree of compatibility as with susceptible barleys. Learn more: https://doi.org/10.1094/PDIS-03-25-0472-RE
Fig. 1. Map showing collection sites of Hordeum jubatum accessions used in this study.
November 14, 2025 at 9:05 PM
plantdiseasej.bsky.social
Jiling Wang, Liyan Zhu, and Chuan-qing Zhang found Alternaria alternata on Fritillaria thunbergii has developed serious resistance to dicarboximide fungicides. Learn more: https://doi.org/10.1094/PDIS-12-24-2638-RE 🌼
November 13, 2025 at 9:30 PM
plantdiseasej.bsky.social
Read “Development of a Tetraplex Reverse Transcription Digital PCR Assay for Three Carlaviruses and an Ilarvirus Infecting Hop and Viral Titer Quantification in Different Tissue Types,” by Natalia Herrera-Blitman et al. in Vol. 109, No. 9 of Plant Disease: https://doi.org/10.1094/PDIS-11-24-2351-RE
Fig. 4. Digital PCR chip images of synthetic control containing corresponding sequences of Hop mosaic virus (blue/FAM), American hop latent virus (green/VIC), Apple mosaic virus (yellow/AvBY), and Hop latent virus (red/JUN) screened with the tetraplex assay at 23 ng/μl (top row) and with established, optimal dilution to 0.00023 ng/μl (bottom row).
November 12, 2025 at 7:55 PM
plantdiseasej.bsky.social
Three viruses causing mosaic-like symptoms in sugarcane and related grasses have been reported in Louisiana since 1919. Dimitre Mollov et al. suggest an Asian origin of sorghum mosaic virus infecting sugarcane in Louisiana and the Western Hemisphere: https://doi.org/10.1094/PDIS-11-24-2359-RE
Fig. 1. Sugarcane leaves exhibiting mosaic symptoms in Louisiana. A, Variety NCo310 infected by sorghum mosaic virus (SrMV) isolate LA-M2009. B, Variety HoCP14-885 infected by SrMV isolate LA-M2029. C, Variety CP65-357 infected by SrMV isolate LA-M2001.
November 11, 2025 at 5:14 PM
plantdiseasej.bsky.social
Rice stripe virus disease, transmitted by the small brown planthopper, is a devastating disease of rice in eastern Asia. Takuya Shiba et al. found the combination of nursery box and field insecticide applications was the most effective at disease control: https://doi.org/10.1094/PDIS-08-24-1707-RE
Fig. 1. Layout of the test site and test plot design in 2013 and 2014 surveys. In 2013, 12 test plots (two fields × six test plots) were established. Clothianidin was applied to nursery boxes at transplanting on 17 May, and silafluofen was applied to the rice field on 2 July (46 days after transplanting). In 2014, eight test plots (two fields × four test plots) were established. Clothianidin was applied to nursery boxes at transplanting on 14 May, and silafluofen was applied to the rice field on 30 June (47 days after transplanting).
November 10, 2025 at 4:53 PM
plantdiseasej.bsky.social
“Synergistic Effect and Water-Dispersible Tablet of Propamidine and Flusilazole Against Botrytis cinerea,” by Yonghui Qiao et al. Read now in Vol. 109, No. 9 of Plant Disease: https://doi.org/10.1094/PDIS-01-25-0153-RE
Fig. 3. Protective and curative activities of the propamidine flusilazole water-dispersible tablet (Pro ⋅ Flu WDT) against Botrytis cinerea on tomato fruits.
November 7, 2025 at 9:07 PM
plantdiseasej.bsky.social
Fusarium crown rot adversely affects wheat production worldwide. Yanqiu He et al. investigated the distribution and diversity of Fusarium spp. collected from wheat samples in 12 regions of Anhui Province, China, in 2020, 2022, and 2024. Learn more: https://doi.org/10.1094/PDIS-01-25-0182-RE
Fig. 1. The diseased wheat samples with symptoms of Fusarium crown rot in different regions were collected in Anhui Province, China.
November 6, 2025 at 9:24 PM
plantdiseasej.bsky.social
Jing Jin et al. accidentally discovered that pyridaben was able to prevent cucumber powdery mildew. The research team explored the inhibitory effects and underlying mechanisms of pyridaben against Sphaerotheca fuliginea: https://doi.org/10.1094/PDIS-11-24-2483-RE
Fig. 6. Effects of 20 mg/liter of pyridaben on hyphal growth and conidial formation of Sphaerotheca fuliginea. S. fuliginea grown on cucumber leaves treated with pyridaben at 0 mg/liter (A and B) and 20 mg/liter (C and D).
November 5, 2025 at 7:54 PM
plantdiseasej.bsky.social
Results from Ori Molad et al. may indicate that different mechanisms are involved in the regulation of severe shoestring-like symptoms induced by ToBRFV-A134T compared with symptoms induced by ToBRFV-WT. Learn more: https://doi.org/10.1094/PDIS-07-24-1533-RE
Fig. 6. Predicted secondary and 3D structures of the MP of ToBRFV-WT, ToBRFV-A134T, TMV-WT, and TMV-N135T. A, A predicted secondary structure and 3D structure of ToBRFV-WT MP (accession no. APB87945.1). B, A predicted secondary structure and 3D structure of ToBRFV-A134T MP. C, A predicted secondary structure and 3D structure of TMV-WT MP (accession no. MH595920). D, A predicted secondary structure and 3D structure of TMV-N135T MP. In the secondary structures, helices are marked as H; strands are marked as A or B arrows; motifs are marked as β-turns, γ-turns, and β-hairpin (a loop). The arrows marked amino acid 134 in ToBRFV and 135 in TMV; the yellow-colored domain marked the 130-to-185 amino acid domain. ToBRFV, tomato brown rugose fruit virus; TMV, tobacco mosaic virus.
November 4, 2025 at 5:14 PM
plantdiseasej.bsky.social
“Identification, Characterization, Pathogenicity, and Host Range of Slafractonia indigofera sp. nov. (Dothidotthiaceae) Causing Leaf Spot Disease of Indigofera pseudotinctoria in China,” by Shan Xu et al. Read now in Vol. 109, No. 9 of Plant Disease: https://doi.org/10.1094/PDIS-01-25-0104-RE
Fig. 1. Symptoms of leaf spot disease on Indigofera pseudotinctoria. A, Infected plants occurred frequently in the fields. B, Typical symptoms of leaf spot on leaflets and petioles. C, Chlorotic leaves with leaf spots after disease progression. D, Leaflet with dark, sunken, necrotic, oval to circular lesions. E, Dark mycelium on black, coalesced, necrotic, irregular-shaped
November 3, 2025 at 5:06 PM