Mike Tisza
@miketisza.bsky.social
Reposted by Mike Tisza
🚨vConTACT3 preprint live!🚨(Peer Review soon...!)
vConTACT3 delivers a unified, scalable, and transparent framework for genome-based virus taxonomy — helping translate big viral data into systematic classification.
🔗 Read the preprint: doi.org/10.1101/2025...
Improvements details below 👇
vConTACT3 delivers a unified, scalable, and transparent framework for genome-based virus taxonomy — helping translate big viral data into systematic classification.
🔗 Read the preprint: doi.org/10.1101/2025...
Improvements details below 👇
Scalable and systematic hierarchical virus taxonomy with vConTACT3
Viruses are key players in diverse ecosystems, but studying their impacts is technically and taxonomically challenging. Taxonomic complexities derive from undersampling, diverse DNA and RNA genomes wi...
doi.org
November 7, 2025 at 4:36 PM
🚨vConTACT3 preprint live!🚨(Peer Review soon...!)
vConTACT3 delivers a unified, scalable, and transparent framework for genome-based virus taxonomy — helping translate big viral data into systematic classification.
🔗 Read the preprint: doi.org/10.1101/2025...
Improvements details below 👇
vConTACT3 delivers a unified, scalable, and transparent framework for genome-based virus taxonomy — helping translate big viral data into systematic classification.
🔗 Read the preprint: doi.org/10.1101/2025...
Improvements details below 👇
Reposted by Mike Tisza
🚨New preprint out!
We present a foundational genomic resource of human gut microbiome viruses. It delivers high-quality, deeply curated data spanning taxonomy, predicted hosts, structures, and functions, providing a reference for gut virome research. (1/8)
www.biorxiv.org/content/10.1...
We present a foundational genomic resource of human gut microbiome viruses. It delivers high-quality, deeply curated data spanning taxonomy, predicted hosts, structures, and functions, providing a reference for gut virome research. (1/8)
www.biorxiv.org/content/10.1...
November 6, 2025 at 5:26 PM
🚨New preprint out!
We present a foundational genomic resource of human gut microbiome viruses. It delivers high-quality, deeply curated data spanning taxonomy, predicted hosts, structures, and functions, providing a reference for gut virome research. (1/8)
www.biorxiv.org/content/10.1...
We present a foundational genomic resource of human gut microbiome viruses. It delivers high-quality, deeply curated data spanning taxonomy, predicted hosts, structures, and functions, providing a reference for gut virome research. (1/8)
www.biorxiv.org/content/10.1...
Reposted by Mike Tisza
Can you take a quarter cup of composite sewage, simply ask ‘what’s in there?’, and find out all of the pathogens circulating in that community?
That is the question we asked in our latest pre-print.
Turns out you can.
1/
www.medrxiv.org/content/10.1...
That is the question we asked in our latest pre-print.
Turns out you can.
1/
www.medrxiv.org/content/10.1...
Untargeted longitudinal ultra deep metagenomic sequencing of wastewater provides a comprehensive readout of expected and unexpected viral pathogens
Wastewater surveillance has become a powerful tool to monitor circulating viruses at a community level. Currently, most wastewater surveillance efforts use target-based approaches such as quantitative...
www.medrxiv.org
October 31, 2025 at 12:19 PM
Can you take a quarter cup of composite sewage, simply ask ‘what’s in there?’, and find out all of the pathogens circulating in that community?
That is the question we asked in our latest pre-print.
Turns out you can.
1/
www.medrxiv.org/content/10.1...
That is the question we asked in our latest pre-print.
Turns out you can.
1/
www.medrxiv.org/content/10.1...
Reposted by Mike Tisza
We're thrilled to announce SeqHub, an AI-enabled platform for biological sequence analysis. SeqHub brings together sequence search, genome annotation, and data sharing in one place.
October 28, 2025 at 1:47 PM
We're thrilled to announce SeqHub, an AI-enabled platform for biological sequence analysis. SeqHub brings together sequence search, genome annotation, and data sharing in one place.
Reposted by Mike Tisza
Excited to share our LongTrack study out in
@natmicrobiol.nature.com today!
Fecal microbiota transplant (FMT), donor 💩 => patients' gut, is an effective treatment for recurrent C. difficile infection & is being evaluated for Inflammatory Bowel Diseases (IBD) & other conditions 1/
📄 rdcu.be/eL8mR
@natmicrobiol.nature.com today!
Fecal microbiota transplant (FMT), donor 💩 => patients' gut, is an effective treatment for recurrent C. difficile infection & is being evaluated for Inflammatory Bowel Diseases (IBD) & other conditions 1/
📄 rdcu.be/eL8mR
Long-read metagenomics for strain tracking after faecal microbiota transplant
Nature Microbiology - A long-read metagenomics method empowers faecal microbiota transplantation studies by precisely tracking bacteria from donors to recipients, distinguishing co-existing strains...
rdcu.be
October 22, 2025 at 3:39 PM
Excited to share our LongTrack study out in
@natmicrobiol.nature.com today!
Fecal microbiota transplant (FMT), donor 💩 => patients' gut, is an effective treatment for recurrent C. difficile infection & is being evaluated for Inflammatory Bowel Diseases (IBD) & other conditions 1/
📄 rdcu.be/eL8mR
@natmicrobiol.nature.com today!
Fecal microbiota transplant (FMT), donor 💩 => patients' gut, is an effective treatment for recurrent C. difficile infection & is being evaluated for Inflammatory Bowel Diseases (IBD) & other conditions 1/
📄 rdcu.be/eL8mR
Reposted by Mike Tisza
New paper out!
We sampled indoor air for a year in a Belgian daycare and used shotgun metagenomics to track viruses. We recovered many viral genomes of interest.
What would you sample next?
Read at @eurosurveillance.org
@emmanuel-microb.bsky.social, @jellematthijnssens.bsky.social
-->
We sampled indoor air for a year in a Belgian daycare and used shotgun metagenomics to track viruses. We recovered many viral genomes of interest.
What would you sample next?
Read at @eurosurveillance.org
@emmanuel-microb.bsky.social, @jellematthijnssens.bsky.social
-->
September 26, 2025 at 1:18 PM
New paper out!
We sampled indoor air for a year in a Belgian daycare and used shotgun metagenomics to track viruses. We recovered many viral genomes of interest.
What would you sample next?
Read at @eurosurveillance.org
@emmanuel-microb.bsky.social, @jellematthijnssens.bsky.social
-->
We sampled indoor air for a year in a Belgian daycare and used shotgun metagenomics to track viruses. We recovered many viral genomes of interest.
What would you sample next?
Read at @eurosurveillance.org
@emmanuel-microb.bsky.social, @jellematthijnssens.bsky.social
-->
Reposted by Mike Tisza
Wastewater Sequencing Reveals Persistent Circulation and Rising Prevalence of Several Oncogenic Viruses Across Texas https://www.medrxiv.org/content/10.1101/2025.09.17.25335998v1
September 18, 2025 at 5:59 PM
Wastewater Sequencing Reveals Persistent Circulation and Rising Prevalence of Several Oncogenic Viruses Across Texas https://www.medrxiv.org/content/10.1101/2025.09.17.25335998v1
Reposted by Mike Tisza
How do you long-read sequence metagenomes? I would argue it starts with the right sample storage & DNA extraction, to enable efficient @nanoporetech.com /@pacbio.bsky.social sequencing, which we investigated in our new paper: www.biorxiv.org/content/10.1...
Massive thanks to Klara for driving this
Massive thanks to Klara for driving this
September 9, 2025 at 3:35 PM
How do you long-read sequence metagenomes? I would argue it starts with the right sample storage & DNA extraction, to enable efficient @nanoporetech.com /@pacbio.bsky.social sequencing, which we investigated in our new paper: www.biorxiv.org/content/10.1...
Massive thanks to Klara for driving this
Massive thanks to Klara for driving this
Reposted by Mike Tisza
Preprint out for myloasm, our new nanopore / HiFi metagenome assembler!
Nanopore's getting accurate, but
1. Can this lead to better metagenome assemblies?
2. How, algorithmically, to leverage them?
with co-author Max Marin @mgmarin.bsky.social, supervised by Heng Li @lh3lh3.bsky.social
1 / N
Nanopore's getting accurate, but
1. Can this lead to better metagenome assemblies?
2. How, algorithmically, to leverage them?
with co-author Max Marin @mgmarin.bsky.social, supervised by Heng Li @lh3lh3.bsky.social
1 / N
High-resolution metagenome assembly for modern long reads with myloasm https://www.biorxiv.org/content/10.1101/2025.09.05.674543v1
September 7, 2025 at 11:35 PM
Preprint out for myloasm, our new nanopore / HiFi metagenome assembler!
Nanopore's getting accurate, but
1. Can this lead to better metagenome assemblies?
2. How, algorithmically, to leverage them?
with co-author Max Marin @mgmarin.bsky.social, supervised by Heng Li @lh3lh3.bsky.social
1 / N
Nanopore's getting accurate, but
1. Can this lead to better metagenome assemblies?
2. How, algorithmically, to leverage them?
with co-author Max Marin @mgmarin.bsky.social, supervised by Heng Li @lh3lh3.bsky.social
1 / N
Reposted by Mike Tisza
A BLAST update adding support for compressed files and csv output with headers is a Good Friday night surprise!
blast.ncbi.nlm.nih.gov/doc/blast-ne...
blast.ncbi.nlm.nih.gov/doc/blast-ne...
2025 BLAST NEWS — BlastNews 0.1.1 documentation
blast.ncbi.nlm.nih.gov
September 6, 2025 at 4:53 AM
A BLAST update adding support for compressed files and csv output with headers is a Good Friday night surprise!
blast.ncbi.nlm.nih.gov/doc/blast-ne...
blast.ncbi.nlm.nih.gov/doc/blast-ne...
I'm very excited to share our manuscript for Cenote-Taker 3. We show that (1) CT3 quickly and accurately annotates previously uncatalogued virus genomes and (2) finds divergent virus genomes in contiguous assemblies. Virome benchmarks are SO hard, but I think we came up with clever and useful ones.
August 25, 2025 at 2:36 PM
I'm very excited to share our manuscript for Cenote-Taker 3. We show that (1) CT3 quickly and accurately annotates previously uncatalogued virus genomes and (2) finds divergent virus genomes in contiguous assemblies. Virome benchmarks are SO hard, but I think we came up with clever and useful ones.
Can anyone explain how MetaROR and Peer Community In are different? @metaror.bsky.social @peercommunityin.bsky.social
(both seem great)
(both seem great)
We are proud to announce a new agreement with @rorinstitute.bsky.social and @aimosinc.bsky.social to become a partner journal with MetaResearch Open Review (MetaRoR) @metaror.bsky.social
#metaresearch #openscience 🧪
Learn more: plos.io/45wPZB9
#metaresearch #openscience 🧪
Learn more: plos.io/45wPZB9
PLOS Biology announces agreement to become a MetaROR partner journal - The Official PLOS Blog
Note: PLOS issued the following press release on Thursday, August 14th. SAN FRANCISCO, CA — Today, PLOS Biology announced a new agreement…
plos.io
August 14, 2025 at 5:41 PM
Can anyone explain how MetaROR and Peer Community In are different? @metaror.bsky.social @peercommunityin.bsky.social
(both seem great)
(both seem great)
Reposted by Mike Tisza
Do you love phage? I have an opening for a postdoc in my lab at the University of South Florida College of Marine Science (@cmarinescience.bsky.social) looking at interactions between marine phage and iron, which is an important limiting trace metal in the oceans (1/5) 🧵#phagesky 🦠🌊
August 1, 2025 at 1:45 PM
Do you love phage? I have an opening for a postdoc in my lab at the University of South Florida College of Marine Science (@cmarinescience.bsky.social) looking at interactions between marine phage and iron, which is an important limiting trace metal in the oceans (1/5) 🧵#phagesky 🦠🌊
Reposted by Mike Tisza
Excited to share a new preprint from the lab with @ryandhindsa.bsky.social ! www.biorxiv.org/content/10.1...
Led by @sherrynyeo.bsky.social, @erinmayc.bsky.social, and friends, we continue our journey to find viral DNA in our favorite place-- the overlooked and discarded reads in existing data! 1/
Led by @sherrynyeo.bsky.social, @erinmayc.bsky.social, and friends, we continue our journey to find viral DNA in our favorite place-- the overlooked and discarded reads in existing data! 1/
July 22, 2025 at 9:59 PM
Excited to share a new preprint from the lab with @ryandhindsa.bsky.social ! www.biorxiv.org/content/10.1...
Led by @sherrynyeo.bsky.social, @erinmayc.bsky.social, and friends, we continue our journey to find viral DNA in our favorite place-- the overlooked and discarded reads in existing data! 1/
Led by @sherrynyeo.bsky.social, @erinmayc.bsky.social, and friends, we continue our journey to find viral DNA in our favorite place-- the overlooked and discarded reads in existing data! 1/
Reposted by Mike Tisza
New pre-print out \o/ All about CRISPR, metagenomes, and what you learn when you collect (a lot of) spacers from natural communities, with @apcamargo.bsky.social @urineri.bsky.social @lhug.bsky.social but also Uri Gophna, Nikhil George (not on Bsky I think) & others at JGI doi.org/10.1101/2025...
doi.org
June 13, 2025 at 8:22 PM
New pre-print out \o/ All about CRISPR, metagenomes, and what you learn when you collect (a lot of) spacers from natural communities, with @apcamargo.bsky.social @urineri.bsky.social @lhug.bsky.social but also Uri Gophna, Nikhil George (not on Bsky I think) & others at JGI doi.org/10.1101/2025...
Reposted by Mike Tisza
Join my lab as a postdoctoral research scientist to investigate the diversity of drug-resistant #Salmonella and how #bacteriophages can help future food safety.
Apply by 2 July, details below!
Apply by 2 July, details below!
🆕 Vacancy! We’re looking for a Research Scientist to join Dr @evelienadri.bsky.social 's group to study bacteriophages, drug resistance and genetic evolution of Salmonella 🦠
💷 £36,720 to £39,750
🗓️ Apply by 2 July 2025
➡️ buff.ly/7M2mDaa
💷 £36,720 to £39,750
🗓️ Apply by 2 July 2025
➡️ buff.ly/7M2mDaa
May 29, 2025 at 9:44 AM
Join my lab as a postdoctoral research scientist to investigate the diversity of drug-resistant #Salmonella and how #bacteriophages can help future food safety.
Apply by 2 July, details below!
Apply by 2 July, details below!
Reposted by Mike Tisza
I'm happy to announce the latest release of the GlobDB, available at globdb.org.
The GlobDB is a database of "species dereplicated" microbial genomes, and as of release 226 contains twice the number of species-representative genomes (306,260) than the latest GTDB release.
The GlobDB is a database of "species dereplicated" microbial genomes, and as of release 226 contains twice the number of species-representative genomes (306,260) than the latest GTDB release.
home | GlobDB
globdb.org
June 10, 2025 at 11:20 AM
I'm happy to announce the latest release of the GlobDB, available at globdb.org.
The GlobDB is a database of "species dereplicated" microbial genomes, and as of release 226 contains twice the number of species-representative genomes (306,260) than the latest GTDB release.
The GlobDB is a database of "species dereplicated" microbial genomes, and as of release 226 contains twice the number of species-representative genomes (306,260) than the latest GTDB release.
Reposted by Mike Tisza
Announcing myloasm, a new long-read (ONT R10/PacBio) metagenome assembler that I've been working on during my postdoc in the Heng Li lab (@lh3lh3.bsky.social).
myloasm-docs.github.io
myloasm-docs.github.io
myloasm - metagenomic assembly with (noisy) long reads
myloasm-docs.github.io
May 28, 2025 at 5:54 PM
Announcing myloasm, a new long-read (ONT R10/PacBio) metagenome assembler that I've been working on during my postdoc in the Heng Li lab (@lh3lh3.bsky.social).
myloasm-docs.github.io
myloasm-docs.github.io
Reposted by Mike Tisza
An innovative outbreak detection program that tracks viruses in #wastewater identified the #measles virus in #Houston in early January 2025, before cases were reported. #TailorLabs #saracregeen @miketisza.bsky.social @bcmhouston.bsky.social #TEPHITexas #RiceU @apha.org www.bcm.edu/news/measles...
May 13, 2025 at 3:46 PM
An innovative outbreak detection program that tracks viruses in #wastewater identified the #measles virus in #Houston in early January 2025, before cases were reported. #TailorLabs #saracregeen @miketisza.bsky.social @bcmhouston.bsky.social #TEPHITexas #RiceU @apha.org www.bcm.edu/news/measles...
Reposted by Mike Tisza
Another example of how wastewater sequencing is going to positively impact Public Health. TEPHI's Outbreak Detection program shows once more the sensitivity and specificity of agnostic sequencing .... ajph.aphapublications.org/doi/abs/10.2...
May 9, 2025 at 12:12 AM
Another example of how wastewater sequencing is going to positively impact Public Health. TEPHI's Outbreak Detection program shows once more the sensitivity and specificity of agnostic sequencing .... ajph.aphapublications.org/doi/abs/10.2...
Reposted by Mike Tisza
Recombination and the Species Structure of the Genus Bacteroides www.biorxiv.org/content/10.1... #jcampubs
May 8, 2025 at 1:16 PM
Recombination and the Species Structure of the Genus Bacteroides www.biorxiv.org/content/10.1... #jcampubs
Reposted by Mike Tisza
🎉🎉 Happy to share our new paper! 🎉🎉
Have you ever wondered about the animal viruses living right alongside us in our cities?
Between our pets at home, rats in the sewers, pigeons on the streets, insects everywhere, and even farm animals nearby, it’s no surprise +++
Have you ever wondered about the animal viruses living right alongside us in our cities?
Between our pets at home, rats in the sewers, pigeons on the streets, insects everywhere, and even farm animals nearby, it’s no surprise +++
April 29, 2025 at 6:30 PM
🎉🎉 Happy to share our new paper! 🎉🎉
Have you ever wondered about the animal viruses living right alongside us in our cities?
Between our pets at home, rats in the sewers, pigeons on the streets, insects everywhere, and even farm animals nearby, it’s no surprise +++
Have you ever wondered about the animal viruses living right alongside us in our cities?
Between our pets at home, rats in the sewers, pigeons on the streets, insects everywhere, and even farm animals nearby, it’s no surprise +++
Reposted by Mike Tisza
I am very happy (and anxious) to share with you our most recent work in which we evaluated four of the most popular long-read assemblers,
www.biorxiv.org/content/10.1...
and tell you just a little bit about it in the following 🧵
www.biorxiv.org/content/10.1...
and tell you just a little bit about it in the following 🧵
Assemblies of long-read metagenomes suffer from diverse errors
Genomes from metagenomes have revolutionised our understanding of microbial diversity, ecology, and evolution, propelling advances in basic science, biomedicine, and biotechnology. Assembly algorithms...
www.biorxiv.org
April 28, 2025 at 8:08 AM
I am very happy (and anxious) to share with you our most recent work in which we evaluated four of the most popular long-read assemblers,
www.biorxiv.org/content/10.1...
and tell you just a little bit about it in the following 🧵
www.biorxiv.org/content/10.1...
and tell you just a little bit about it in the following 🧵
Reposted by Mike Tisza
🧪🧪🧪10 incredible findings about Cas10-relative, mCpol:
result number 10 will surprise you!🧪🧪🧪
@erinedoherty.bsky.social and I teamed up to understand the role and function of Cas10-relative, mCpol, and its role in antiphage immunity. For more, check out Erin's thread 👇
result number 10 will surprise you!🧪🧪🧪
@erinedoherty.bsky.social and I teamed up to understand the role and function of Cas10-relative, mCpol, and its role in antiphage immunity. For more, check out Erin's thread 👇
Preprint alert! ✨ In this project that I co-led with @benadler.bsky.social, we show that a miniature CRISPR-Cas10-like enzyme, mCpol, uses a novel inverse signaling mechanism to prevent the spread of viruses that attempt immune evasion by depleting host cyclic nucleotides.
Check it out:
Check it out:
A miniature CRISPR-Cas10 enzyme confers immunity by an inverse signaling pathway
Microbial and viral co-evolution has created immunity mechanisms involving oligonucleotide signaling that share mechanistic features with human anti-viral systems. In these pathways, including CBASS a...
www.biorxiv.org
March 31, 2025 at 5:31 PM
🧪🧪🧪10 incredible findings about Cas10-relative, mCpol:
result number 10 will surprise you!🧪🧪🧪
@erinedoherty.bsky.social and I teamed up to understand the role and function of Cas10-relative, mCpol, and its role in antiphage immunity. For more, check out Erin's thread 👇
result number 10 will surprise you!🧪🧪🧪
@erinedoherty.bsky.social and I teamed up to understand the role and function of Cas10-relative, mCpol, and its role in antiphage immunity. For more, check out Erin's thread 👇
Reposted by Mike Tisza
Comparative wastewater virome analysis with different enrichment methods https://www.biorxiv.org/content/10.1101/2025.03.25.645222v1
March 26, 2025 at 2:16 PM
Comparative wastewater virome analysis with different enrichment methods https://www.biorxiv.org/content/10.1101/2025.03.25.645222v1