Luke Chao
@lukechao.bsky.social
Exploring membrane ultrastructure and dynamics
chaolab.org
chaolab.org
Reposted by Luke Chao
Out in print! Using ancestral sequence reconstruction 🦖, in vivo characterization, and 🔬 analysis, we identified key residues in the mitochondrial protein #Mic60 that affect #respiration in #yeast.
Since the preprint, we added some cool findings. 🧵⬇️
onlinelibrary.wiley.com/doi/10.1002/...
Since the preprint, we added some cool findings. 🧵⬇️
onlinelibrary.wiley.com/doi/10.1002/...
Ancestral sequence reconstruction of the Mic60 Mitofilin domain reveals residues supporting respiration in yeast
In eukaryotes, cellular respiration takes place in the cristae of mitochondria. The mitochondrial inner membrane protein Mic60, a core component of the mitochondrial contact site and cristae organizi...
onlinelibrary.wiley.com
June 23, 2025 at 9:44 PM
Out in print! Using ancestral sequence reconstruction 🦖, in vivo characterization, and 🔬 analysis, we identified key residues in the mitochondrial protein #Mic60 that affect #respiration in #yeast.
Since the preprint, we added some cool findings. 🧵⬇️
onlinelibrary.wiley.com/doi/10.1002/...
Since the preprint, we added some cool findings. 🧵⬇️
onlinelibrary.wiley.com/doi/10.1002/...
Reposted by Luke Chao
I’m thrilled to share that I will join @stowersinstitute.bsky.social this fall to explore the evolution and design of bioenergetic membranes in bacteria.
Meet Friederike Benning, PhD, the newest Jim and Virginia Stowers Fellow! Her research dives into the complexity of energy generation in evolution to reveal how bacteria build membranes to survive in extreme environments. 🦠
Learn more: bit.ly/3SCZQiM
Learn more: bit.ly/3SCZQiM
May 16, 2025 at 12:57 PM
I’m thrilled to share that I will join @stowersinstitute.bsky.social this fall to explore the evolution and design of bioenergetic membranes in bacteria.
Reposted by Luke Chao
Meet Friederike Benning, PhD, the newest Jim and Virginia Stowers Fellow! Her research dives into the complexity of energy generation in evolution to reveal how bacteria build membranes to survive in extreme environments. 🦠
Learn more: bit.ly/3SCZQiM
Learn more: bit.ly/3SCZQiM
May 15, 2025 at 6:27 PM
Meet Friederike Benning, PhD, the newest Jim and Virginia Stowers Fellow! Her research dives into the complexity of energy generation in evolution to reveal how bacteria build membranes to survive in extreme environments. 🦠
Learn more: bit.ly/3SCZQiM
Learn more: bit.ly/3SCZQiM
Congrats Chen and colleagues (with shoutout to @myfry.bsky.social for connecting our labs)! JCI - SARM1 loss protects retinal ganglion cells in a mouse model of Autosomal Dominant Optic Atrophy www.jci.org/articles/vie...
JCI -
SARM1 loss protects retinal ganglion cells in a mouse model of Autosomal Dominant Optic Atrophy
www.jci.org
May 10, 2025 at 2:52 PM
Congrats Chen and colleagues (with shoutout to @myfry.bsky.social for connecting our labs)! JCI - SARM1 loss protects retinal ganglion cells in a mouse model of Autosomal Dominant Optic Atrophy www.jci.org/articles/vie...
Reposted by Luke Chao
It has been coming a long way... happy to share our latest work with you: www.biorxiv.org/content/10.1...
Here, we show the importance of septal fortification by the Class A Penicillin Binding Protein, PBP1b, in E. coli and provide a molecular mechanism for this function. (1/12).
Here, we show the importance of septal fortification by the Class A Penicillin Binding Protein, PBP1b, in E. coli and provide a molecular mechanism for this function. (1/12).
The aPBP-type cell wall synthase PBP1b plays a specialized role in fortifying the Escherichia coli division site against osmotic rupture
A multi-protein system called the divisome promotes bacterial division. This apparatus synthesizes the peptidoglycan (PG) cell wall layer that forms the daughter cell poles and protects them from osmo...
www.biorxiv.org
April 3, 2025 at 10:13 PM
It has been coming a long way... happy to share our latest work with you: www.biorxiv.org/content/10.1...
Here, we show the importance of septal fortification by the Class A Penicillin Binding Protein, PBP1b, in E. coli and provide a molecular mechanism for this function. (1/12).
Here, we show the importance of septal fortification by the Class A Penicillin Binding Protein, PBP1b, in E. coli and provide a molecular mechanism for this function. (1/12).
Reposted by Luke Chao
A new #preLight has appeared! ✨🔔
Barbora highlights a preprinted study from the Chao Lab (@lukechao.bsky.social) in which the structure-function relationship at the site of cellular respiration is under review. 👀
Check it out! ⬇️
prelights.biologists.com/highlights/a...
Barbora highlights a preprinted study from the Chao Lab (@lukechao.bsky.social) in which the structure-function relationship at the site of cellular respiration is under review. 👀
Check it out! ⬇️
prelights.biologists.com/highlights/a...
Ancestral sequence reconstruction of the Mic60 Mitofilin domain reveals residues supporting respiration in yeast - preLights
In this preprint the structure-function relationship at the site of cellular respiration is under review.
prelights.biologists.com
March 11, 2025 at 12:18 PM
A new #preLight has appeared! ✨🔔
Barbora highlights a preprinted study from the Chao Lab (@lukechao.bsky.social) in which the structure-function relationship at the site of cellular respiration is under review. 👀
Check it out! ⬇️
prelights.biologists.com/highlights/a...
Barbora highlights a preprinted study from the Chao Lab (@lukechao.bsky.social) in which the structure-function relationship at the site of cellular respiration is under review. 👀
Check it out! ⬇️
prelights.biologists.com/highlights/a...
Reposted by Luke Chao
Great summary of our recent preprint by Barbora from @prelights.bsky.social. #mitochondria #cristae #respiration #asr
A new #preLight has appeared! ✨🔔
Barbora highlights a preprinted study from the Chao Lab (@lukechao.bsky.social) in which the structure-function relationship at the site of cellular respiration is under review. 👀
Check it out! ⬇️
prelights.biologists.com/highlights/a...
Barbora highlights a preprinted study from the Chao Lab (@lukechao.bsky.social) in which the structure-function relationship at the site of cellular respiration is under review. 👀
Check it out! ⬇️
prelights.biologists.com/highlights/a...
Ancestral sequence reconstruction of the Mic60 Mitofilin domain reveals residues supporting respiration in yeast - preLights
In this preprint the structure-function relationship at the site of cellular respiration is under review.
prelights.biologists.com
March 11, 2025 at 1:07 PM
Great summary of our recent preprint by Barbora from @prelights.bsky.social. #mitochondria #cristae #respiration #asr
Reposted by Luke Chao
Excited to share our collaborative work characterizing a pathogenic mutation in Opa1 found in ADOA patients that began when Tom Schwarz
@BostonChildrens
presented a seminar
@MGHMolBio
biorxiv.org/content/10.1...
@lukechao.bsky.social
@BostonChildrens
presented a seminar
@MGHMolBio
biorxiv.org/content/10.1...
@lukechao.bsky.social
Loss of SARM1 protects against retinal ganglion cell degeneration in Autosomal Dominant Optic Atrophy
Autosomal Dominant Optic Atrophy (ADOA), the most prevalent inherited optic neuropathy, leads to retinal ganglion cell (RGC) degeneration and vision loss. ADOA is primarily caused by mutations in the ...
biorxiv.org
October 3, 2024 at 1:57 PM
Excited to share our collaborative work characterizing a pathogenic mutation in Opa1 found in ADOA patients that began when Tom Schwarz
@BostonChildrens
presented a seminar
@MGHMolBio
biorxiv.org/content/10.1...
@lukechao.bsky.social
@BostonChildrens
presented a seminar
@MGHMolBio
biorxiv.org/content/10.1...
@lukechao.bsky.social
Congrats to Siva Boopathy and team: www.sciencedirect.com/science/arti...
Identification of SLC25A46 interaction interfaces with mitochondrial membrane fusogens Opa1 and Mfn2
Mitochondrial fusion requires the sequential merger of four bilayers to two. The outer-membrane solute carrier protein SLC25A46 interacts with both th…
www.sciencedirect.com
September 9, 2024 at 2:16 PM
Congrats to Siva Boopathy and team: www.sciencedirect.com/science/arti...
Happy to see our work out in
@elife.bsky.social ! Lead by Tristan Bell and team. #EVs doi.org/10.7554/eLif...
@elife.bsky.social ! Lead by Tristan Bell and team. #EVs doi.org/10.7554/eLif...
Prominin 1 and Tweety Homology 1 both induce extracellular vesicle formation
doi.org
August 13, 2024 at 9:06 PM
Happy to see our work out in
@elife.bsky.social ! Lead by Tristan Bell and team. #EVs doi.org/10.7554/eLif...
@elife.bsky.social ! Lead by Tristan Bell and team. #EVs doi.org/10.7554/eLif...
Reposted by Luke Chao
How do baculoviruses protect their genome?
Delighted to present our findings in print!
We use cryo-EM to determine the structure of a baculoviral nucleocapsid. We show how the tiny capsid protein VP39 assembles into a giant nucleocapsid.❄️🔬
www.nature.com/articles/s41...
Delighted to present our findings in print!
We use cryo-EM to determine the structure of a baculoviral nucleocapsid. We show how the tiny capsid protein VP39 assembles into a giant nucleocapsid.❄️🔬
www.nature.com/articles/s41...
Helical reconstruction of VP39 reveals principles for baculovirus nucleocapsid assembly - Nature Com...
Baculoviruses are used as insecticides, gene therapy tools, and protein production vectors. Here, the authors report the structure of the Autographa californica MNPV nucleocapsid and its fold conserva...
www.nature.com
January 8, 2024 at 9:44 AM
How do baculoviruses protect their genome?
Delighted to present our findings in print!
We use cryo-EM to determine the structure of a baculoviral nucleocapsid. We show how the tiny capsid protein VP39 assembles into a giant nucleocapsid.❄️🔬
www.nature.com/articles/s41...
Delighted to present our findings in print!
We use cryo-EM to determine the structure of a baculoviral nucleocapsid. We show how the tiny capsid protein VP39 assembles into a giant nucleocapsid.❄️🔬
www.nature.com/articles/s41...
January 4, 2024 at 6:19 PM
Great way to ring in the New Year from Siva Boopathy and colleagues:
Identification of SLC25A46 interaction interfaces with mitochondrial membrane fusogens Mfn2 and Opa1 www.biorxiv.org/content/10.1...
Identification of SLC25A46 interaction interfaces with mitochondrial membrane fusogens Mfn2 and Opa1 www.biorxiv.org/content/10.1...
December 30, 2023 at 12:23 AM
Great way to ring in the New Year from Siva Boopathy and colleagues:
Identification of SLC25A46 interaction interfaces with mitochondrial membrane fusogens Mfn2 and Opa1 www.biorxiv.org/content/10.1...
Identification of SLC25A46 interaction interfaces with mitochondrial membrane fusogens Mfn2 and Opa1 www.biorxiv.org/content/10.1...