King Cada
@kingcada.bsky.social
Studying the molecular sociology between cellular membranes and organelles, in neurons, with the proteins that shape and transport them
Our lab’s paper spearheaded by Keni and pushed to completion (and satisfaction by the reviewers) by Satish is finally out www.nature.com/articles/s41.... Was so fortunate to contribute to this project and learned a lot in both neuronal cell biology and cryoET. Also, Satish is on the job market!
November 12, 2025 at 5:35 PM
Our lab’s paper spearheaded by Keni and pushed to completion (and satisfaction by the reviewers) by Satish is finally out www.nature.com/articles/s41.... Was so fortunate to contribute to this project and learned a lot in both neuronal cell biology and cryoET. Also, Satish is on the job market!
Reposted by King Cada
ICYMI: New online: Architecture of the human KICSTOR and GATOR1–KICSTOR complexes
Architecture of the human KICSTOR and GATOR1–KICSTOR complexes
Nature Structural & Molecular Biology, Published online: 06 November 2025; doi:10.1038/s41594-025-01693-4Teng and Zeng et al. use cryo-electron microscopy to show that the crescent scaffold of KICSTOR anchors GATOR1 to lysosomes and disruption of the interaction causes mTORC1 hyperactivation and TFE3 mislocalization. KICSTOR enables nutrient-dependent mTORC1 regulation by binding anionic lipids for lysosomal targeting.
go.nature.com
November 7, 2025 at 11:45 AM
ICYMI: New online: Architecture of the human KICSTOR and GATOR1–KICSTOR complexes
Reposted by King Cada
Finally got to go through this carefully. An interesting paper and a tremendous amount of work.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling
Synaptic vesicle (SV) exocytosis underpins neuronal communication, yet its nanoscale dynamics remain poorly understood owing to limitations in visualizing rapid events in situ. Here, we used optogenet...
www.science.org
November 7, 2025 at 9:50 PM
Finally got to go through this carefully. An interesting paper and a tremendous amount of work.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by King Cada
Excited to share our study out in @natcellbio.nature.com! Led by @mikelangelipid.bsky.social, we identify the first #LipidDroplet lipid quality control pathway: LD-localized FSP1 protects stored lipids from oxidative damage and prevents LD-initiated #ferroptosis.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
FSP1-mediated lipid droplet quality control prevents neutral lipid peroxidation and ferroptosis - Nature Cell Biology
Lange et al. identify a lipid droplet quality control pathway in which FSP1 safeguards stored neutral lipids from lipid peroxidation, thereby preventing the induction of ferroptosis.
www.nature.com
October 29, 2025 at 5:21 PM
Excited to share our study out in @natcellbio.nature.com! Led by @mikelangelipid.bsky.social, we identify the first #LipidDroplet lipid quality control pathway: LD-localized FSP1 protects stored lipids from oxidative damage and prevents LD-initiated #ferroptosis.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by King Cada
warmest congratulations to Scott Emr and Wes Sundquist for being awarded the 2025 World Laureate Association Prize in life science or medicine english.shanghai.gov.cn/en-Latest-Ta...
WLA doesnt do a great job advertizing in the US so i am doing their job for them. So well deserved!
WLA doesnt do a great job advertizing in the US so i am doing their job for them. So well deserved!
World Laureates Association announces 2025 prize winners_Latest
The World Laureates Association (WLA) announced its laureates for 2025 in Shanghai on Wednesday, recognizing groundbreaking contributions in the fields of life and mathematical sciences.
english.shanghai.gov.cn
October 28, 2025 at 12:03 AM
warmest congratulations to Scott Emr and Wes Sundquist for being awarded the 2025 World Laureate Association Prize in life science or medicine english.shanghai.gov.cn/en-Latest-Ta...
WLA doesnt do a great job advertizing in the US so i am doing their job for them. So well deserved!
WLA doesnt do a great job advertizing in the US so i am doing their job for them. So well deserved!
Reposted by King Cada
First neurons didn’t appear overnight. We trace their roots to ancient secretory cells - showing how lifestyle & behavior shaped the evolution of first synapses.🧠🌊 #Evolution #Neuroscience
Our latest in @natrevneuro.nature.com
Link: rdcu.be/eMX3E
@jeffcolgren.bsky.social @msarscentre.bsky.social
Our latest in @natrevneuro.nature.com
Link: rdcu.be/eMX3E
@jeffcolgren.bsky.social @msarscentre.bsky.social
The evolutionary origins of synaptic proteins and their changing roles in different organisms across evolution
Nature Reviews Neuroscience - Recent studies have shed further light on the evolutionary origins of chemical synapses, In this Review, Colgren and Burkhardt explore how ancient proteins were...
rdcu.be
October 27, 2025 at 6:48 PM
First neurons didn’t appear overnight. We trace their roots to ancient secretory cells - showing how lifestyle & behavior shaped the evolution of first synapses.🧠🌊 #Evolution #Neuroscience
Our latest in @natrevneuro.nature.com
Link: rdcu.be/eMX3E
@jeffcolgren.bsky.social @msarscentre.bsky.social
Our latest in @natrevneuro.nature.com
Link: rdcu.be/eMX3E
@jeffcolgren.bsky.social @msarscentre.bsky.social
Reposted by King Cada
An efficient eukaryotic cell-free expression and correlative cryo-electron tomography platform for structural biology of macromolecular complexes www.biorxiv.org/content/10.1101/2025.10.18.683253v1 #cryoem
October 21, 2025 at 7:10 AM
An efficient eukaryotic cell-free expression and correlative cryo-electron tomography platform for structural biology of macromolecular complexes www.biorxiv.org/content/10.1101/2025.10.18.683253v1 #cryoem
Reposted by King Cada
Visualization of lysosomal membrane proteins by cryo electron tomography pubmed.ncbi.nlm.nih.gov/41107240/ #cryoem
October 18, 2025 at 6:41 PM
Visualization of lysosomal membrane proteins by cryo electron tomography pubmed.ncbi.nlm.nih.gov/41107240/ #cryoem
Reposted by King Cada
As ever, beautiful #cryoEM work from the lab of Hong Zhou with field-defining insights using #cryoET:
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science
www.science.org/doi/10.1126/...
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science
www.science.org/doi/10.1126/...
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling
Synaptic vesicle (SV) exocytosis underpins neuronal communication, yet its nanoscale dynamics remain poorly understood owing to limitations in visualizing rapid events in situ. Here, we used optogenet...
www.science.org
October 16, 2025 at 10:34 PM
As ever, beautiful #cryoEM work from the lab of Hong Zhou with field-defining insights using #cryoET:
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science
www.science.org/doi/10.1126/...
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science
www.science.org/doi/10.1126/...
Reposted by King Cada
Now out in Science! Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. In @vcushing.bsky.social's magnum opus, we use #cryoEM to figure out how the CDK-activating kinase recognises CDKs to fully activate them - a key step in cell cycle control.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Structural basis of T-loop–independent recognition and activation of CDKs by the CDK-activating kinase
Cyclin-dependent kinases (CDKs) are prototypical regulators of the cell cycle. The CDK-activating kinase (CAK) acts as a master regulator of CDK activity by catalyzing the activating phosphorylation o...
www.science.org
October 16, 2025 at 7:17 PM
Now out in Science! Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. In @vcushing.bsky.social's magnum opus, we use #cryoEM to figure out how the CDK-activating kinase recognises CDKs to fully activate them - a key step in cell cycle control.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by King Cada
🧠 The Lipid #Brain Atlas is out now! If you think #lipids are boring and membranes are all the same, prepare to be surprised. Led by @lucafusarbassini.bsky.social with Giovanni D'Angelo's lab, we mapped membrane lipids in the mouse brain at high resolution.
www.biorxiv.org/cgi/content/...
www.biorxiv.org/cgi/content/...
October 16, 2025 at 6:23 AM
🧠 The Lipid #Brain Atlas is out now! If you think #lipids are boring and membranes are all the same, prepare to be surprised. Led by @lucafusarbassini.bsky.social with Giovanni D'Angelo's lab, we mapped membrane lipids in the mouse brain at high resolution.
www.biorxiv.org/cgi/content/...
www.biorxiv.org/cgi/content/...
Reposted by King Cada
See you there! Will try to throw a cytoskeleton party for my birthday 🎇
Learn all things #cytoskeleton #research in an interdisciplinary environment at EMBO Workshop "Structure and Function of the Cytoskeleton" in Paris, FR, 7–10 April 2026.
Deadline: 20 December 2025
https://meetings.embo.org/event/26-cytoskeleton
#EMBOCytoskeletalFunction #EMBOevents #conference 🧪
Deadline: 20 December 2025
https://meetings.embo.org/event/26-cytoskeleton
#EMBOCytoskeletalFunction #EMBOevents #conference 🧪
Structure and Function of the Cytoskeleton
The cytoskeleton, a dynamic and intricate network of protein filaments, is found within the cytoplasm of all cells, from bacteria and archaea to complex eukaryotes. This essential cellular component …
meetings.embo.org
October 7, 2025 at 8:53 PM
See you there! Will try to throw a cytoskeleton party for my birthday 🎇
Reposted by King Cada
Beautiful live-cell imaging of lysosome tubulation along microtubules in this work just out from @lysoluis.bsky.social et al:
rupress.org/jcb/article/...
rupress.org/jcb/article/...
September 26, 2025 at 6:14 AM
Beautiful live-cell imaging of lysosome tubulation along microtubules in this work just out from @lysoluis.bsky.social et al:
rupress.org/jcb/article/...
rupress.org/jcb/article/...
Reposted by King Cada
Using cryo-ET, CRN Team Reck-Peterson showed that full-length LRRK2 can oligomerize on microtubules in its autoinhibited state. Both WT-LRRK2 and PD-linked LRRK2 mutants formed filaments on microtubules.
🔗 Read the full #preprint to learn more about their recent research: bit.ly/3VrVNaB
🔗 Read the full #preprint to learn more about their recent research: bit.ly/3VrVNaB
September 26, 2025 at 3:29 PM
Using cryo-ET, CRN Team Reck-Peterson showed that full-length LRRK2 can oligomerize on microtubules in its autoinhibited state. Both WT-LRRK2 and PD-linked LRRK2 mutants formed filaments on microtubules.
🔗 Read the full #preprint to learn more about their recent research: bit.ly/3VrVNaB
🔗 Read the full #preprint to learn more about their recent research: bit.ly/3VrVNaB
Reposted by King Cada
Our new preprint is up! This is the main postdoc work of @wiesner-t.bsky.social focusing on exocytosis along the axon shaft and its regulation by the sub membrane actin-spectrin scaffold: www.biorxiv.org/content/10.1...
Read the thread below for a summary of our findings 🧵1/11
Read the thread below for a summary of our findings 🧵1/11
September 17, 2025 at 2:55 PM
Our new preprint is up! This is the main postdoc work of @wiesner-t.bsky.social focusing on exocytosis along the axon shaft and its regulation by the sub membrane actin-spectrin scaffold: www.biorxiv.org/content/10.1...
Read the thread below for a summary of our findings 🧵1/11
Read the thread below for a summary of our findings 🧵1/11
Woot! Congrats Chen!
Finally in @nature.com 🎉
We uncovered step-wise mTORC1 activation on membrane: RAG–Ragulator recruits, RHEB pushes closer, and direct membrane contacts by RAPTOR + mTOR trigger full kinase activity.👉 The membrane is not just a platform — it’s part of the mechanism.
www.nature.com/articles/s41...
We uncovered step-wise mTORC1 activation on membrane: RAG–Ragulator recruits, RHEB pushes closer, and direct membrane contacts by RAPTOR + mTOR trigger full kinase activity.👉 The membrane is not just a platform — it’s part of the mechanism.
www.nature.com/articles/s41...
Structural basis for mTORC1 activation on the lysosomal membrane - Nature
Cryo-electron microscopy was used to study human mechanistic target of rapamycin complex 1 (mTORC1) activation on lysosomal membranes, showing progressive recruitment by RAG–Ragulator, RHEB and R...
www.nature.com
September 18, 2025 at 4:26 PM
Woot! Congrats Chen!
Reposted by King Cada
Structural basis for mTORC1 activation on the lysosomal membrane pubmed.ncbi.nlm.nih.gov/40963021/ #cryoem
September 18, 2025 at 4:22 PM
Structural basis for mTORC1 activation on the lysosomal membrane pubmed.ncbi.nlm.nih.gov/40963021/ #cryoem
Great couple of days working with colleagues at NIEHS and Duke!
September 18, 2025 at 2:25 AM
Great couple of days working with colleagues at NIEHS and Duke!
Reposted by King Cada
I'm very excited to share out latest work now published in Autophagy.
www.tandfonline.com/doi/full/10....
This work was led by Dr. Devika Andhare and demonstrates the membrane binding and clustering capabilities of Atg11 using a new reconstitution system.
www.tandfonline.com/doi/full/10....
This work was led by Dr. Devika Andhare and demonstrates the membrane binding and clustering capabilities of Atg11 using a new reconstitution system.
Reconstitution of autophagic-like membrane tethering reveals that Atg11 can bind and cluster vesicles on cargo mimetics
Macroautophagy (hereafter, autophagy) is essential for the degradation of mitochondria from yeast to humans. Mitochondrial autophagy in yeast is initiated when the selective autophagy scaffolding p...
www.tandfonline.com
September 3, 2025 at 12:48 PM
I'm very excited to share out latest work now published in Autophagy.
www.tandfonline.com/doi/full/10....
This work was led by Dr. Devika Andhare and demonstrates the membrane binding and clustering capabilities of Atg11 using a new reconstitution system.
www.tandfonline.com/doi/full/10....
This work was led by Dr. Devika Andhare and demonstrates the membrane binding and clustering capabilities of Atg11 using a new reconstitution system.
Reposted by King Cada
Excited to share our new review with @depaceraffa.bsky.social, @saikat2025.bsky.social, and Chad Williamson on the BLOC-1 and BORC complexes—key regulators of endolysosomal processes and linked to several genetic diseases. #NIH #Lysosomes #RareDiseases authors.elsevier.com/a/1lg4i8jWWJ...
August 26, 2025 at 3:30 PM
Excited to share our new review with @depaceraffa.bsky.social, @saikat2025.bsky.social, and Chad Williamson on the BLOC-1 and BORC complexes—key regulators of endolysosomal processes and linked to several genetic diseases. #NIH #Lysosomes #RareDiseases authors.elsevier.com/a/1lg4i8jWWJ...
Reposted by King Cada
👉 New publication ahead of print from our lab: tinyurl.com/ye7pntte
We show that the protein SPG21, mutated in hereditary spastic paraplegia 21, localizes to endolysosomes via RAB7A, where it promotes mTORC1-dependent TFEB phosphorylation, reducing expression of a subset of TFEB regulated genes
We show that the protein SPG21, mutated in hereditary spastic paraplegia 21, localizes to endolysosomes via RAB7A, where it promotes mTORC1-dependent TFEB phosphorylation, reducing expression of a subset of TFEB regulated genes
August 21, 2025 at 6:03 PM
👉 New publication ahead of print from our lab: tinyurl.com/ye7pntte
We show that the protein SPG21, mutated in hereditary spastic paraplegia 21, localizes to endolysosomes via RAB7A, where it promotes mTORC1-dependent TFEB phosphorylation, reducing expression of a subset of TFEB regulated genes
We show that the protein SPG21, mutated in hereditary spastic paraplegia 21, localizes to endolysosomes via RAB7A, where it promotes mTORC1-dependent TFEB phosphorylation, reducing expression of a subset of TFEB regulated genes
Reposted by King Cada
big one! a decade in the making. nadler & team develop tools to track lipid transport in living cells at single-species resolution. turns out non-vesicular, asymmetry-driven transport is the main architect of organelle identity. feels like the dawn of a golden era for lipid biology. rdcu.be/eBGJv
Quantitative imaging of lipid transport in mammalian cells
Nature - Directional, non-vesicular lipid transport is responsible for fast, species-selective lipid sorting into organelle membranes.
rdcu.be
August 21, 2025 at 8:51 AM
big one! a decade in the making. nadler & team develop tools to track lipid transport in living cells at single-species resolution. turns out non-vesicular, asymmetry-driven transport is the main architect of organelle identity. feels like the dawn of a golden era for lipid biology. rdcu.be/eBGJv
Reposted by King Cada
In situ cryo-electron tomography of vaccinia virus exit from infected cells https://www.biorxiv.org/content/10.1101/2025.08.20.671218v1
August 20, 2025 at 11:30 AM
In situ cryo-electron tomography of vaccinia virus exit from infected cells https://www.biorxiv.org/content/10.1101/2025.08.20.671218v1
Reposted by King Cada
Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH
A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo
A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo
Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug
Nature Structural & Molecular Biology - Integrated structural biology approach leveraging in situ cryo-electron tomography reveals molecular details of the native malarial translation...
rdcu.be
August 18, 2025 at 12:13 PM
Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH
A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo
A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo
Reposted by King Cada
Repost appreciated:
Open #PhDposition in structural virology in my group! Join us in Umeå, Sweden to uncover how arboviruses remodel infected cells. In situ #cryoET and #cryoEM combined with #virology, #cellbiology, and #biophysics.
Deadline 7 September. More info: www.carlsonlab.se/join/
Open #PhDposition in structural virology in my group! Join us in Umeå, Sweden to uncover how arboviruses remodel infected cells. In situ #cryoET and #cryoEM combined with #virology, #cellbiology, and #biophysics.
Deadline 7 September. More info: www.carlsonlab.se/join/
August 13, 2025 at 10:10 PM
Repost appreciated:
Open #PhDposition in structural virology in my group! Join us in Umeå, Sweden to uncover how arboviruses remodel infected cells. In situ #cryoET and #cryoEM combined with #virology, #cellbiology, and #biophysics.
Deadline 7 September. More info: www.carlsonlab.se/join/
Open #PhDposition in structural virology in my group! Join us in Umeå, Sweden to uncover how arboviruses remodel infected cells. In situ #cryoET and #cryoEM combined with #virology, #cellbiology, and #biophysics.
Deadline 7 September. More info: www.carlsonlab.se/join/