Kazuhiro Maeshima
@kazu-maeshima.bsky.social
#Chromatin biologist/biophysicist @NIG & SOKENDAI in Japan.
Chromatin is very dynamic and flexible, but NOT regular!!! π§ͺπ§¬π¬
My career and work: http://bit.ly/2CuF4L5γ
Lab HP: https://bit.ly/3F1a8nk
YouTube Seminar: https://bit.ly/4fYZiOj
Chromatin is very dynamic and flexible, but NOT regular!!! π§ͺπ§¬π¬
My career and work: http://bit.ly/2CuF4L5γ
Lab HP: https://bit.ly/3F1a8nk
YouTube Seminar: https://bit.ly/4fYZiOj
Pinned
My student Aoi Otsuka βs PhD work is now officially published @csf-jscb.bsky.social! π¬ Single-nucleosome imaging uncovers biphasic local chromatin dynamics in an oncogene-inducible human carcinogenesis model (1β3 d: same, 5β7 d: β, back by week 4). Congrats! www.jstage.jst.go.jp/article/csf/...
Fascinating cryo-ET study! π¬π§¬
We can now trace strings of nucleosomes directly in cells.
Congratulations to the @eltsovmikhail.bsky.socialβ¬ team! π
We can now trace strings of nucleosomes directly in cells.
Congratulations to the @eltsovmikhail.bsky.socialβ¬ team! π
We are in EMBO J. See DNA and non-octameric nucleosome-like particles in situ
link.springer.com/article/10.1...
link.springer.com/article/10.1...
Direct visualization and tracing of chromatin folding in the Drosophila embryo - The EMBO Journal
Chromatin organization, through the assembly of DNA with histones and the folding of nucleosome chains, regulates DNA accessibility for transcription, DNA replication and repair. Although models deriv...
link.springer.com
February 11, 2026 at 8:55 AM
Fascinating cryo-ET study! π¬π§¬
We can now trace strings of nucleosomes directly in cells.
Congratulations to the @eltsovmikhail.bsky.socialβ¬ team! π
We can now trace strings of nucleosomes directly in cells.
Congratulations to the @eltsovmikhail.bsky.socialβ¬ team! π
My student Aoi Otsuka βs PhD work is now officially published @csf-jscb.bsky.social! π¬ Single-nucleosome imaging uncovers biphasic local chromatin dynamics in an oncogene-inducible human carcinogenesis model (1β3 d: same, 5β7 d: β, back by week 4). Congrats! www.jstage.jst.go.jp/article/csf/...
February 10, 2026 at 1:19 PM
My student Aoi Otsuka βs PhD work is now officially published @csf-jscb.bsky.social! π¬ Single-nucleosome imaging uncovers biphasic local chromatin dynamics in an oncogene-inducible human carcinogenesis model (1β3 d: same, 5β7 d: β, back by week 4). Congrats! www.jstage.jst.go.jp/article/csf/...
@tadasu443.bsky.social was a PhD student @nigidenken.bsky.social (2012β2017). Our papers together π§¬β¨:
www.cell.com/molecular-ce...
www.science.org/doi/full/10....
www.cell.com/molecular-ce...
www.science.org/doi/full/10....
Dynamic Organization of Chromatin Domains Revealed by Super-Resolution Live-Cell Imaging
How a genome is organized and behaves in live cells remains unclear. Nozaki etΒ al.
visualized little bunches of chromatin, βchromatin domains,β and their dynamic behavior
in live mammalian cells. The ...
www.cell.com
January 12, 2026 at 5:19 AM
@tadasu443.bsky.social was a PhD student @nigidenken.bsky.social (2012β2017). Our papers together π§¬β¨:
www.cell.com/molecular-ce...
www.science.org/doi/full/10....
www.cell.com/molecular-ce...
www.science.org/doi/full/10....
Thank you for your kind words. Please visit us next time.
January 8, 2026 at 1:37 PM
Thank you for your kind words. Please visit us next time.
Happy New Year! π
Todayβs view of Mt. Fuji over Mishima City β @nigidenken.bsky.social is on the right.
Wishing you all a wonderful year!
Todayβs view of Mt. Fuji over Mishima City β @nigidenken.bsky.social is on the right.
Wishing you all a wonderful year!
January 4, 2026 at 7:48 AM
Happy New Year! π
Todayβs view of Mt. Fuji over Mishima City β @nigidenken.bsky.social is on the right.
Wishing you all a wonderful year!
Todayβs view of Mt. Fuji over Mishima City β @nigidenken.bsky.social is on the right.
Wishing you all a wonderful year!
Reposted by Kazuhiro Maeshima
Weβre recruiting PhD students. If youβre interested in chromosome biology or imaging, please apply to the MCB or Plant Biology graduate programs at UMass (deadline is soon, 12/1). DM me for projects or questions.
www.umass.edu/molecular-ce...
www.umass.edu/plant-biolog...
www.umass.edu/molecular-ce...
www.umass.edu/plant-biolog...
How to Apply : Molecular & Cellular Biology : UMass Amherst
A bachelor's degree from a qualified college or university is required or expected prior to the program's start date. Applicants are expected to have taken college-level courses in Biology, Organic Ch...
www.umass.edu
November 13, 2025 at 12:13 PM
Weβre recruiting PhD students. If youβre interested in chromosome biology or imaging, please apply to the MCB or Plant Biology graduate programs at UMass (deadline is soon, 12/1). DM me for projects or questions.
www.umass.edu/molecular-ce...
www.umass.edu/plant-biolog...
www.umass.edu/molecular-ce...
www.umass.edu/plant-biolog...
So proud of my former PhD student βͺ@tadasu443.bsky.socialβ¬ for starting his own lab at @umassamherst.bsky.social ! Wishing him all the best in this exciting new chapter. Looking forward to his future discoveries! ππ§¬
I launched my lab at UMass Amherst this fall. We study the mechanisms of meiotic homolog pairing using advanced liveβcell and superβresolution imaging. Currently in budding yeast, with plans to expand to other eukaryotes. #Chromosome #Meiosis #Mitosis
www.umass.edu/biology/abou...
www.umass.edu/biology/abou...
December 28, 2025 at 10:20 AM
So proud of my former PhD student βͺ@tadasu443.bsky.socialβ¬ for starting his own lab at @umassamherst.bsky.social ! Wishing him all the best in this exciting new chapter. Looking forward to his future discoveries! ππ§¬
We deeply appreciate our collaborators π: @katsuminami.bsky.social and Tamura-san @nigidenken.bsky.social; Kiyono-sensei @NCCRI @Sasaki Institute; Watanabe-san and Takeshita-sansei @NCCRI
doi.org/10.1247/csf....
doi.org/10.1247/csf....
December 23, 2025 at 2:34 AM
We deeply appreciate our collaborators π: @katsuminami.bsky.social and Tamura-san @nigidenken.bsky.social; Kiyono-sensei @NCCRI @Sasaki Institute; Watanabe-san and Takeshita-sansei @NCCRI
doi.org/10.1247/csf....
doi.org/10.1247/csf....
My PhD students Aoi Otsuka @masaashimazoe.bsky.social et al. published @csf-jscb.bsky.social π Single-nucleosome imaging in an oncogene-inducible human carcinogenesis model shows biphasic chromatin dynamics (1β3 d same; 5β7 d β; back by week 4). Congrats! π
doi.org/10.1247/csf....
doi.org/10.1247/csf....
December 23, 2025 at 2:34 AM
My PhD students Aoi Otsuka @masaashimazoe.bsky.social et al. published @csf-jscb.bsky.social π Single-nucleosome imaging in an oncogene-inducible human carcinogenesis model shows biphasic chromatin dynamics (1β3 d same; 5β7 d β; back by week 4). Congrats! π
doi.org/10.1247/csf....
doi.org/10.1247/csf....
Reposted by Kazuhiro Maeshima
Our Science paper is out!
Huge congratulations to @huabin-zhou.bsky.social, Mike Rosen, and the brilliant @janhuemar.bsky.social @juliamaristany.bsky.social and @kieran-russell.bsky.social from our group
News: bit.ly/4avnkAr and bit.ly/3XBGVHS
Great perspective by @vram142.bsky.social +K Zhang
Huge congratulations to @huabin-zhou.bsky.social, Mike Rosen, and the brilliant @janhuemar.bsky.social @juliamaristany.bsky.social and @kieran-russell.bsky.social from our group
News: bit.ly/4avnkAr and bit.ly/3XBGVHS
Great perspective by @vram142.bsky.social +K Zhang
December 5, 2025 at 9:47 AM
Our Science paper is out!
Huge congratulations to @huabin-zhou.bsky.social, Mike Rosen, and the brilliant @janhuemar.bsky.social @juliamaristany.bsky.social and @kieran-russell.bsky.social from our group
News: bit.ly/4avnkAr and bit.ly/3XBGVHS
Great perspective by @vram142.bsky.social +K Zhang
Huge congratulations to @huabin-zhou.bsky.social, Mike Rosen, and the brilliant @janhuemar.bsky.social @juliamaristany.bsky.social and @kieran-russell.bsky.social from our group
News: bit.ly/4avnkAr and bit.ly/3XBGVHS
Great perspective by @vram142.bsky.social +K Zhang
Reposted by Kazuhiro Maeshima
Excited to share our latest fully in-house paper! π
We show how cohesin integrates loop extrusion with sister tethering to guide the homology search during DNA repair.
Huge congratulations to all authors, and to @fedeteloni.bsky.social for leading this work π
Weβre excited to see his next steps!
We show how cohesin integrates loop extrusion with sister tethering to guide the homology search during DNA repair.
Huge congratulations to all authors, and to @fedeteloni.bsky.social for leading this work π
Weβre excited to see his next steps!
I am happy to share that my postdoctoral work in the @gerlichlab.bsky.social at @imbavienna.bsky.social is finally out π!
Our study reveals how cohesin guides focused and accurate homology search.
Read more π www.science.org/doi/10.1126/...
Follow along for key insights and updates! π§΅
Our study reveals how cohesin guides focused and accurate homology search.
Read more π www.science.org/doi/10.1126/...
Follow along for key insights and updates! π§΅
Cohesin guides homology search during DNA repair using loops and sister chromatid linkages
Accurate repair of DNA double-strand breaks (DSBs) is essential for genome stability, and defective repair underlies diseases such as cancer. Homologous recombination uses an intact homologous sequenc...
www.science.org
December 5, 2025 at 7:46 AM
Excited to share our latest fully in-house paper! π
We show how cohesin integrates loop extrusion with sister tethering to guide the homology search during DNA repair.
Huge congratulations to all authors, and to @fedeteloni.bsky.social for leading this work π
Weβre excited to see his next steps!
We show how cohesin integrates loop extrusion with sister tethering to guide the homology search during DNA repair.
Huge congratulations to all authors, and to @fedeteloni.bsky.social for leading this work π
Weβre excited to see his next steps!
Exciting paper @natcomms.nature.com from Kanemaki lab @nigidenken.bsky.socialγCongratulations ππ
nature.com/articles/s41...
Using a ligase-depletion OK-seq approach, they map DNA replication initiation zones genome-wide in human cells and identify TRESLINβMTBP as a key limiting firing factor.
nature.com/articles/s41...
Using a ligase-depletion OK-seq approach, they map DNA replication initiation zones genome-wide in human cells and identify TRESLINβMTBP as a key limiting firing factor.
Regulated TRESLIN-MTBP loading governs initiation zones and replication timing in human DNA replication - Nature Communications
DNA replication in the human genome occurs preferentially at initiation zones (IZs). Here, the authors identify TRESLIN-MTBP as a limiting factor for replication initiation whose loading onto DNA-boun...
nature.com
December 4, 2025 at 12:49 AM
Exciting paper @natcomms.nature.com from Kanemaki lab @nigidenken.bsky.socialγCongratulations ππ
nature.com/articles/s41...
Using a ligase-depletion OK-seq approach, they map DNA replication initiation zones genome-wide in human cells and identify TRESLINβMTBP as a key limiting firing factor.
nature.com/articles/s41...
Using a ligase-depletion OK-seq approach, they map DNA replication initiation zones genome-wide in human cells and identify TRESLINβMTBP as a key limiting firing factor.
Reposted by Kazuhiro Maeshima
Our recent paper offers insights into unligated Okazaki fragment by the absence of Rad27/FEN-1 leading to severe rDNA copy number changes.
doi.org/10.1002/1873...
doi.org/10.1002/1873...
FEBS Press
The budding yeast Rad27 is a structure-specific endonuclease. Here, the authors reveal that Rad27 is crucial for maintaining the stability of the ribosomal RNA gene (rDNA) region. Rad27 deficiency le....
doi.org
November 27, 2025 at 9:53 AM
Our recent paper offers insights into unligated Okazaki fragment by the absence of Rad27/FEN-1 leading to severe rDNA copy number changes.
doi.org/10.1002/1873...
doi.org/10.1002/1873...
Reposted by Kazuhiro Maeshima
The paper from our lab came out on how the histone chaperone CAF-1 prevents the formation of circular rDNA!
doi.org/10.1093/nar/...
doi.org/10.1093/nar/...
The histone chaperone CAF-1 prevents homologous recombination-mediated instability of the budding yeast ribosomal DNA during replication-coupled DNA double-strand break repair
Abstract. DNA replication-coupled chromatin assembly is crucial to maintain genome integrity. Here, we demonstrate that the absence of the budding yeast hi
doi.org
November 27, 2025 at 9:41 AM
The paper from our lab came out on how the histone chaperone CAF-1 prevents the formation of circular rDNA!
doi.org/10.1093/nar/...
doi.org/10.1093/nar/...
Reposted by Kazuhiro Maeshima
Super-resolution single-molecule fluorescence combined with quantitative phase contrast. This powerful combo enables ~0.05 nm optical path difference detection, < 20 nm resolution, and continuous live-cell imaging with digital staining
doi.org/10.1101/2025...
#Microscopy #CellBiology #OI-DIC #SMLM
doi.org/10.1101/2025...
#Microscopy #CellBiology #OI-DIC #SMLM
A correlative quantitative phase contrast and fluorescence super-resolution microscope for imaging molecules in their cellular context
Fluorescence microscopy has been widely used to reveal the spatial distribution of specifically labeled molecules, but it is blind to cellular context. Quantitative phase contrast microscopy (QPC) pro...
doi.org
October 16, 2025 at 11:57 PM
Super-resolution single-molecule fluorescence combined with quantitative phase contrast. This powerful combo enables ~0.05 nm optical path difference detection, < 20 nm resolution, and continuous live-cell imaging with digital staining
doi.org/10.1101/2025...
#Microscopy #CellBiology #OI-DIC #SMLM
doi.org/10.1101/2025...
#Microscopy #CellBiology #OI-DIC #SMLM
Reposted by Kazuhiro Maeshima
3rd histone paper on my feed today!
Our new preprint is outοΌ bioRxiv: www.biorxiv.org/content/10.1...
@masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in living cells. π Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and othersβhuge thanks! π 1/
@masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in living cells. π Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and othersβhuge thanks! π 1/
September 11, 2025 at 9:11 PM
3rd histone paper on my feed today!
Thanks. Yes, we agree to your point.
September 11, 2025 at 11:21 PM
Thanks. Yes, we agree to your point.
Thanks @zeinabrekad.bsky.social for your kind words. We totally agree to your point, and indeed we are working on H1:
bsky.app/profile/kazu...
www.biorxiv.org/content/10.1...
bsky.app/profile/kazu...
www.biorxiv.org/content/10.1...
September 11, 2025 at 11:20 PM
Thanks @zeinabrekad.bsky.social for your kind words. We totally agree to your point, and indeed we are working on H1:
bsky.app/profile/kazu...
www.biorxiv.org/content/10.1...
bsky.app/profile/kazu...
www.biorxiv.org/content/10.1...
Thanks! π
September 11, 2025 at 11:12 PM
Thanks! π
Reposted by Kazuhiro Maeshima
Condensed chromatin domain structure and cohesin-constrained mobility assure independent transcription regulation for each domain!
Also consistent with many previous studies.
(4/n)
Also consistent with many previous studies.
(4/n)
August 29, 2025 at 10:57 AM
Condensed chromatin domain structure and cohesin-constrained mobility assure independent transcription regulation for each domain!
Also consistent with many previous studies.
(4/n)
Also consistent with many previous studies.
(4/n)
Reposted by Kazuhiro Maeshima
Euchromatin is mostly condensedβοΈ
With @shiori-iida.bsky.social , We revealed the detailed structure of euchromatin using super-resolution imaging.
Cohesin regulates the function of euchromatin via chromatin dynamics!
(1/n)
With @shiori-iida.bsky.social , We revealed the detailed structure of euchromatin using super-resolution imaging.
Cohesin regulates the function of euchromatin via chromatin dynamics!
(1/n)
Is euchromatin really βopenβ? Our new study @bioRxiv suggests otherwise. Using super-resolution imaging @shiori-iida.bsky.socialβ¬ @masaashimazoe.bsky.social reveals: Euchromatin forms condensed domains in live cells. Cohesin constrains them and prevents domain mixing.
www.biorxiv.org/cgi/content/...
www.biorxiv.org/cgi/content/...
August 28, 2025 at 8:43 AM
Euchromatin is mostly condensedβοΈ
With @shiori-iida.bsky.social , We revealed the detailed structure of euchromatin using super-resolution imaging.
Cohesin regulates the function of euchromatin via chromatin dynamics!
(1/n)
With @shiori-iida.bsky.social , We revealed the detailed structure of euchromatin using super-resolution imaging.
Cohesin regulates the function of euchromatin via chromatin dynamics!
(1/n)
Reposted by Kazuhiro Maeshima
One more movie!
Using the RL algorithm (from pnas.org/doi/10.1073/...),
@masaashimazoe.bsky.social analyzed our nucleosome tracking data and classified them by their mobility.
Hotter color = faster motion (Slowπ΅β π’ β π‘ β π Fast)
Using the RL algorithm (from pnas.org/doi/10.1073/...),
@masaashimazoe.bsky.social analyzed our nucleosome tracking data and classified them by their mobility.
Hotter color = faster motion (Slowπ΅β π’ β π‘ β π Fast)
August 29, 2025 at 12:36 AM
One more movie!
Using the RL algorithm (from pnas.org/doi/10.1073/...),
@masaashimazoe.bsky.social analyzed our nucleosome tracking data and classified them by their mobility.
Hotter color = faster motion (Slowπ΅β π’ β π‘ β π Fast)
Using the RL algorithm (from pnas.org/doi/10.1073/...),
@masaashimazoe.bsky.social analyzed our nucleosome tracking data and classified them by their mobility.
Hotter color = faster motion (Slowπ΅β π’ β π‘ β π Fast)
Reposted by Kazuhiro Maeshima
Our new preprint is out!
We combined single-nucleosome imaging and 3D-SIM to reveal:
πΉ Euchromatin forms condensed domains, not open fibers
πΉCohesin loss increases nucleosome mobility without decompaction
πΉCohesin prevents neighboring domain mixing
Full story & moviesπ
We combined single-nucleosome imaging and 3D-SIM to reveal:
πΉ Euchromatin forms condensed domains, not open fibers
πΉCohesin loss increases nucleosome mobility without decompaction
πΉCohesin prevents neighboring domain mixing
Full story & moviesπ
Is euchromatin really βopenβ? Our new study @bioRxiv suggests otherwise. Using super-resolution imaging @shiori-iida.bsky.socialβ¬ @masaashimazoe.bsky.social reveals: Euchromatin forms condensed domains in live cells. Cohesin constrains them and prevents domain mixing.
www.biorxiv.org/cgi/content/...
www.biorxiv.org/cgi/content/...
August 28, 2025 at 3:11 PM
Our new preprint is out!
We combined single-nucleosome imaging and 3D-SIM to reveal:
πΉ Euchromatin forms condensed domains, not open fibers
πΉCohesin loss increases nucleosome mobility without decompaction
πΉCohesin prevents neighboring domain mixing
Full story & moviesπ
We combined single-nucleosome imaging and 3D-SIM to reveal:
πΉ Euchromatin forms condensed domains, not open fibers
πΉCohesin loss increases nucleosome mobility without decompaction
πΉCohesin prevents neighboring domain mixing
Full story & moviesπ
Thank you so much!
August 29, 2025 at 10:43 AM
Thank you so much!
Thank you for the nice list. Could you add me to the list?
August 29, 2025 at 8:03 AM
Thank you for the nice list. Could you add me to the list?