Ivan Maslov
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ivanmasl0v.bsky.social
Ivan Maslov
@ivanmasl0v.bsky.social
MSCA Fellow postdoc at the Membrane Enzymology group, University of Groningen; tweeting about fluorescence microscopy, smFRET, membrane proteins, bacteria & GPCRs

https://scholar.google.nl/citations?user=lC_EQr4AAAAJ&hl=en
Pinned
#smFRET-lovers! Imagine, you got nice burst data with freely diffusing molecules 🙌🔬

BUT
1) Immobilization does not work🧲🤬
2) Transitions are longer than diffusion time, but shorter than frametime of your TIRF⏱️

Solution: try ABEL-trap!💕

📜bioRxiv:
doi.org/10.1101/2025.07.22.666123
ABEL-FRET bridges the timescale gap in single-molecule measurements of the structural dynamics in the A2A adenosine receptor
The functional complexity of G protein-coupled receptors (GPCRs) arises from their structural dynamics, which span timescales from nanoseconds to minutes. Single-molecule Förster Resonance Energy Tran...
doi.org
Reposted by Ivan Maslov
🧬 New paper out!
Thrilled to contribute to our study on CaV1.3 channel clusters in cardiac myocytes using DNA-PAINT (4 nm loc. precision!) revealing flexible (not rigid) cluster organization.
👏 Great work by Niko Schwenzer, @samratb.bsky.social & team!
👉 www.sciencedirect.com/science/arti...
November 10, 2025 at 1:06 PM
It was fun to share my passion for science at the #EuropeanResearchersNight in Groningen!
✨ Curious visitors
✅matched 🧫 plates with microbes to where they were collected
✅inspected flowers, berries, and lichens under the microscope🔬
✅ created their own “bacterial selfies”📸
October 2, 2025 at 4:22 PM
#smFRET-lovers! Imagine, you got nice burst data with freely diffusing molecules 🙌🔬

BUT
1) Immobilization does not work🧲🤬
2) Transitions are longer than diffusion time, but shorter than frametime of your TIRF⏱️

Solution: try ABEL-trap!💕

📜bioRxiv:
doi.org/10.1101/2025.07.22.666123
ABEL-FRET bridges the timescale gap in single-molecule measurements of the structural dynamics in the A2A adenosine receptor
The functional complexity of G protein-coupled receptors (GPCRs) arises from their structural dynamics, which span timescales from nanoseconds to minutes. Single-molecule Förster Resonance Energy Tran...
doi.org
July 28, 2025 at 1:27 PM
Reposted by Ivan Maslov
ABEL-FRET bridges the timescale gap in single-molecule measurements of the structural dynamics in the A2A adenosine receptor https://www.biorxiv.org/content/10.1101/2025.07.22.666123v1
July 26, 2025 at 3:47 AM
It was a great Day 1 of the EMBL Symposium "New approaches and concepts in microbiology"! Stepping a little outside of my usual microscopy/biophysics bubble feels like the right kind of challenge - wish me more good ideas, inspiration, and coffee
#EESMicrobiology
June 24, 2025 at 9:16 PM
Reposted by Ivan Maslov
Setting up a lab has been no joke. Today, I'm stoked to have one more piece of my postdoc work out in the wild.
Several more manuscripts to go, and I don't have a lot of time for a blue-torial(?) today, but enjoy and happy to get any feedback on this story.

www.biorxiv.org/content/10.1...
Membrane phosphoinositides allosterically tune β-arrestin dynamics to facilitate GPCR core engagement
Arrestin proteins bind active G protein-coupled receptors (GPCRs) through coordinated protein-protein, protein-phosphate, and protein-lipid interactions to attenuate G protein signaling and promote GP...
www.biorxiv.org
June 10, 2025 at 12:41 AM
⚛️Physics Olympiads hold a special place in my❤️

I cherish memories of my teachers and still enjoy the charm of the intellectual puzzles🥲

This year, I designed a few problems for the Dutch Physics Olympiads ⏳

Congratulations to the winners and good luck at #IPhO2025 🥳
June 11, 2025 at 7:52 AM
Reposted by Ivan Maslov
beautiful pulsed labelings reveal where new wall form as bacteria grow
How does your favorite species elongate? 🧵 "Phenotypic plasticity in bacterial elongation among closely related species". Happy to see this paper in print @natcomms.nature.com. Nice work by @mariedelaby.bsky.social, Liu Yang et al. See original 🧵, different colours, same data and conclusions.
June 3, 2025 at 4:33 AM
🚨 #LLPS: New trick for an old membrane protein!

Homo- and hetero- biomolecular condensates of a native membrane protein in E. coli tagged with PopTag 🔬🦠

LacY-PopTag is functional and localizes at cell poles or mobile foci in a curvature-dependent manner

🔗 doi.org/10.21203/rs.3.rs-6571918/v1
May 26, 2025 at 11:25 AM
Reposted by Ivan Maslov
Giulia Viola, Torsten Wittmann @microtubule.bsky.social and colleagues @ctbatucsf.bsky.social use protein nanocages to benchmark fluorescent proteins in live cells.
Highlight: journals.biologists.com/jcs/article/...
Article: journals.biologists.com/jcs/article/...
#OpenAccess #ReadandPublish
May 23, 2025 at 12:02 PM
Reposted by Ivan Maslov
Solène’s 4.5 years of postdoctoral work in our lab (she started just before the corona pandemic) has now been published. A heroic effort that revealed -at the single molecule level- that ATP drives the dissociation of an ECF transporter complex under turnover conditions. See rdcu.be/elTKc
Single-molecule visualization of ATP-induced dynamics of the subunit composition of an ECF transporter complex under turnover conditions
Nature Communications - The association and dissociation dynamics of the ECF transporter complex for vitamin B12 are visualized by single-molecule FRET, highlighting the original transport...
rdcu.be
May 13, 2025 at 7:30 PM
Reposted by Ivan Maslov
Register: forms.gle/4hDdwKNhXMJj...
Happening TOMORROW!

Join us on April 25 at 7PM EAT for a powerful webinar with @atinygreencell.bsky.social on making synthetic biology accessible to all.
Hosted by @synbio4allafrica.bsky.social .
April 24, 2025 at 7:45 AM
Reposted by Ivan Maslov
We are looking for a postdoc with microbiology background for #superresolution #singlemolecule #ERC tracking project. Apply by 27.4.2025. 🦠🔬🧫
Pls share!

www.aalto.fi/en/open-posi...
April 1, 2025 at 7:30 AM
Reposted by Ivan Maslov
1/8 Exciting new work with Agu @mangiarotti.bsky.social @mpici.bsky.social ! 🚀 Our latest study explores how lipid packing and cholesterol regulate the interaction of biomolecular condensates with membranes. It turns out, it’s not just about membrane phase. Packing is the key! 🧵👇
rdcu.be/eelLq
Lipid packing and cholesterol content regulate membrane wetting and remodeling by biomolecular condensates
Nature Communications - Membrane lipid packing, influenced by cholesterol, lipid chain length, and saturation, regulates the affinity of biomolecular condensates, enhancing their interactions with...
rdcu.be
March 21, 2025 at 8:47 AM
Reposted by Ivan Maslov
New Night Science Podcast episode! David Baker was awarded the 2024 Nobel Prize in Chemistry. He explains how he socially engineers his lab’s "communal brain", where all individuals function like neurons, densely interconnected to maximize idea generation.
nightscience.buzzsprout.com/1744020/epis...
March 25, 2025 at 7:30 AM
Reposted by Ivan Maslov
Protein function often depends on protein dynamics. To design proteins that function like natural ones, how do we predict their dynamics?

@hkws.bsky.social and I are thrilled to share the first big, experimental datasets on protein dynamics and our new model: Dyna-1!

🧵
March 20, 2025 at 3:02 PM
Reposted by Ivan Maslov
A comprehensive review on covalent chemical strategies for protein self-labeling from @kjohnsson.bsky.social's lab: HaloTag, SNAP-tag et al. www.annualreviews.org/content/jour...
March 20, 2025 at 8:56 PM
How is one dye #NileRed (NR) the sensor of both polarity and microviscosity?

Sarkar et al. correlate spectral shifts and fluorophore orientations of single molecules of NR in Lo/Ld supported lipid bilayers

PhysChemB: doi.org/10.1021/acs.jpcb.4c07578
🔓 bioRxiv: doi.org/10.1101/2024.06.21.600028
Spectrally Resolved Single-Molecule Orientation Imaging Reveals a Direct Correspondence between the Polarity and Microviscosity Experienced by Nile Red in Supported Lipid Bilayer Membranes
Molecular-level interactions among lipids, cholesterol, and water dictate the nanoscale membrane organization of lipid bilayers into liquid-ordered (Lo) and liquid-disordered (Ld) phases, characterize...
doi.org
February 28, 2025 at 3:04 PM
Single-molecule microscopy is full of surprises:
see how anisotropy can be used to follow the conformational dynamics! 🤯 #24-state-model

"Examination of conformational dynamics of AdiC transporter with fluorescence-polarization microscopy" @jgp.org 🔥🔬

rupress.org/jgp/article/...
February 26, 2025 at 7:15 PM
Reposted by Ivan Maslov
New paper out 🎇
It has been 13 years since @owenlabuob.bsky.social and co. published a stellar protocol on how to measure membrane order with smart probes! Since then, there has been new dyes, imaging modes and biology! We wrote a protocol for Nature Protocols:
www.nature.com/articles/s41...
Measuring plasma membrane fluidity using confocal microscopy - Nature Protocols
Membrane fluidity is a crucial feature in understanding cellular physiology. This protocol describes a robust pipeline for measuring plasma membrane fluidity using confocal imaging and new environment...
www.nature.com
February 19, 2025 at 3:58 PM
Reposted by Ivan Maslov
Nature research paper: De novo design of transmembrane fluorescence-activating proteins

https://go.nature.com/4k2OAZA
De novo design of transmembrane fluorescence-activating proteins - Nature
A study describes the design of de novo ligand-binding transmembrane proteins, demonstrating their specific binding and activation of fluorogenic ligands.
go.nature.com
February 20, 2025 at 8:46 AM
Reposted by Ivan Maslov
Structure of the F-tractin-F-actin complex. A new study by Dmitry Shatskiy, Athul Sivan, Roland Wedlich-Söldner @uni-muenster.de, and Alexander Belyy @alexanderbelyy.bsky.social @unigroningen.bsky.social: https://buff.ly/3EIqAur

#Cytoskeleton #StructuralBiology #Biochemistry #Actin
February 17, 2025 at 2:07 PM
Reposted by Ivan Maslov
Join me today at ⏰ 9:30AM in Room 404AB 👨‍🏫 for a journey through the 🌎 world of "Spectral RICS - Realtime imaging 🔬 of molecular parameters made easy" at the #BPS2025 meeting (1/3)
February 17, 2025 at 4:15 PM
🚨🔬#preprint :

λ-STED 🔬improves spectral sensitivity with polarity-sensitive probes 🌈 and enables correlative measurements of membrane order and anomalous lipid diffusion

www.biorxiv.org/content/10.1...

Congrats to the authors! 👏👏
@uiztok.bsky.social, @faldalf.bsky.social @sciezgin.bsky.social
Spectral STED microscopy improves spectral sensitivity with polarity-sensitive probes and enables correlative measurements of membrane order and anomalous lipid diffusion
Molecular plasma membrane organization and dynamics play an important role in cellular signalling. Advances in our understanding of the nanoscale architecture of the plasma membrane heavily rely on th...
www.biorxiv.org
February 16, 2025 at 5:41 PM
"Direct effect of membrane environment on the activation of mGluR2 revealed by single-molecule FRET" in #Structure
@cellpress.bsky.social

🔗 doi.org/10.1016/j.str.2025.01.011

🧵 #GPCR #mGluR🧼#detergents #cholesterol
🔬 #smFRET
💻🧪 #MD #mutants

Congrats to C. Banerjee, B. Liauw, and R. Vafabakhsh!
Redirecting
doi.org
February 9, 2025 at 11:54 PM