Harrold van den Burg
@havandenburg.bsky.social
Research manager Biotechnology Rijk Zwaan | Special chair in Plant pathology | University of Amsterdam | Plant Virology | Xanthomonas | Plant disease susceptibility | Messages are my own
Pinned
➡️ New insights into the evolution of the vascular pathogen Xanthomonas campestris involving the genome defense system CRISPR-Cas! www.cell.com/current-biol... Our data wizard 🪄@mpaauw.bsky.social sequenced almost 100 genomes and this is what he found … /
Evolution of a vascular plant pathogen is associated with the loss of CRISPR-Cas and an increase in genome plasticity and virulence genes
Paauw et al. report on the evolution of a plant pathogenic Xanthomonas pathovar and
show that the gain of mobile genetic elements and virulence factors coincided with
the loss of the CRISPR-Cas genome...
www.cell.com
Great new study from our lab revealing insights in Xanthomonas evolution and how loss of the Crispr genome defense was a key step for X. campestris pv campestris.
Reposted by Harrold van den Burg
We wrote a review 📖 on hydathode immunity! Learn how bacteria exploit hydathodes to access the vasculature and open questions for future research.
📣 If you are curious about this topic, please consider applying for two open positions in my lab (until Nov23)! www.sciencedirect.com/science/arti...
📣 If you are curious about this topic, please consider applying for two open positions in my lab (until Nov23)! www.sciencedirect.com/science/arti...
Hydathodes at the forefront of plant immunity against vascular pathogens
Hydathodes are tiny plant organs that form an interface between the leaf surface and xylem vasculature. They facilitate excretion of xylem fluid under…
www.sciencedirect.com
November 4, 2025 at 10:43 AM
We wrote a review 📖 on hydathode immunity! Learn how bacteria exploit hydathodes to access the vasculature and open questions for future research.
📣 If you are curious about this topic, please consider applying for two open positions in my lab (until Nov23)! www.sciencedirect.com/science/arti...
📣 If you are curious about this topic, please consider applying for two open positions in my lab (until Nov23)! www.sciencedirect.com/science/arti...
Reposted by Harrold van den Burg
Very happy to share our latest work “Systematic discovery and engineering of synthetic immune receptors in plants” out in @science.org !
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Systematic discovery and engineering of synthetic immune receptors in plants
Plants deploy a diverse array of pattern recognition receptors (PRRs), which perceive microbe-associated molecular patterns to activate immune responses. Leucine-rich repeat receptor-like kinase subgr...
www.science.org
September 4, 2025 at 7:33 PM
Very happy to share our latest work “Systematic discovery and engineering of synthetic immune receptors in plants” out in @science.org !
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Harrold van den Burg
Finally a method to identify plant RNA-binding proteins! 🧬 This enables so much new biology to discover from immunity to development! 🤯 🪴 Established by the incredible Victor Sanchez with @havandenburg.bsky.social @uva.nl Congratulations!!
Protocol for capturing the RNA-binding proteome from plants using orthogonal organic phase separation https://www.biorxiv.org/content/10.1101/2025.07.29.667348v1
July 29, 2025 at 7:35 PM
Finally a method to identify plant RNA-binding proteins! 🧬 This enables so much new biology to discover from immunity to development! 🤯 🪴 Established by the incredible Victor Sanchez with @havandenburg.bsky.social @uva.nl Congratulations!!
Reposted by Harrold van den Burg
www.nature.com/articles/s41...
Brilliant new strategy to obtain broad resistance against Potyviruses, and possibly other protease-encoding viruses
Brilliant new strategy to obtain broad resistance against Potyviruses, and possibly other protease-encoding viruses
Remodelling autoactive NLRs for broad-spectrum immunity in plants - Nature
Cleavage by pathogen-derived proteases of an engineered chimeric protein activates its plant immune receptor component, enabling broad-spectrum resistance to pathogens in plants.
www.nature.com
July 28, 2025 at 8:44 AM
www.nature.com/articles/s41...
Brilliant new strategy to obtain broad resistance against Potyviruses, and possibly other protease-encoding viruses
Brilliant new strategy to obtain broad resistance against Potyviruses, and possibly other protease-encoding viruses
Reposted by Harrold van den Burg
Hydathode immunity 🌱 - the discovery journey continues ... ⏩ Today: Immune receptor SUT1 confers resistance against #Xanthomonas pathogens in hydathodes. In the latest version, we contrast the tissue-specificity of SUT1 with the well-known ZAR1 resistance.
journals.plos.org/plospathogen...
journals.plos.org/plospathogen...
Arabidopsis CNL receptor SUT1 confers immunity in hydathodes against the vascular pathogen Xanthomonas campestris pv. campestris
Author summary Black rot disease, caused by the bacterial pathogen Xanthomonas campestris pv. campestris (Xcc), is an economically important disease of cabbage crops. Xcc is unique in that it enters t...
journals.plos.org
July 2, 2025 at 6:51 PM
Hydathode immunity 🌱 - the discovery journey continues ... ⏩ Today: Immune receptor SUT1 confers resistance against #Xanthomonas pathogens in hydathodes. In the latest version, we contrast the tissue-specificity of SUT1 with the well-known ZAR1 resistance.
journals.plos.org/plospathogen...
journals.plos.org/plospathogen...
Reposted by Harrold van den Burg
Happy to share that our Helixer deeplearning-based annotation of 230 superasterid genomes is now online on
@datadryad.bsky.social
💻🌱
doi.org/10.5061/drya...
Check out how we used 69 of them to study the molecular evolution of a plant immune receptor network here! 👇
@datadryad.bsky.social
💻🌱
doi.org/10.5061/drya...
Check out how we used 69 of them to study the molecular evolution of a plant immune receptor network here! 👇
May 9, 2025 at 11:19 AM
Happy to share that our Helixer deeplearning-based annotation of 230 superasterid genomes is now online on
@datadryad.bsky.social
💻🌱
doi.org/10.5061/drya...
Check out how we used 69 of them to study the molecular evolution of a plant immune receptor network here! 👇
@datadryad.bsky.social
💻🌱
doi.org/10.5061/drya...
Check out how we used 69 of them to study the molecular evolution of a plant immune receptor network here! 👇
Reposted by Harrold van den Burg
Reciprocal phosphorylation between SOAK1 and SOBIR1 fine-tunes receptor-like protein (RLP)–mediated plant immunity @science.org #ScienceAdvances
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reciprocal phosphorylation between SOAK1 and SOBIR1 fine-tunes receptor-like protein (RLP)–mediated plant immunity
SOAK1-SOBIR1 module fine-tunes receptor-like protein (RLP)–mediated plant immunity.
www.science.org
April 21, 2025 at 2:36 PM
Reciprocal phosphorylation between SOAK1 and SOBIR1 fine-tunes receptor-like protein (RLP)–mediated plant immunity @science.org #ScienceAdvances
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Harrold van den Burg
Huanglongbing causes crop damage worth $10 billion annually, and most trees die within a few years once infected.
This week in a new Science study, researchers report that they have developed therapeutic peptides that may be able to save and revive infected citrus trees. scim.ag/4jtM8dC
This week in a new Science study, researchers report that they have developed therapeutic peptides that may be able to save and revive infected citrus trees. scim.ag/4jtM8dC
April 10, 2025 at 6:15 PM
Huanglongbing causes crop damage worth $10 billion annually, and most trees die within a few years once infected.
This week in a new Science study, researchers report that they have developed therapeutic peptides that may be able to save and revive infected citrus trees. scim.ag/4jtM8dC
This week in a new Science study, researchers report that they have developed therapeutic peptides that may be able to save and revive infected citrus trees. scim.ag/4jtM8dC
Reposted by Harrold van den Burg
Beautiful paper
Exogenous RNA surveillance by proton-sensing TRIM25
Exogenous messenger RNAs (mRNAs) require cellular machinery for delivery and translation but also encounter inhibitory factors. To investigate their regulation, we performed genome-wide CRISPR screens...
www.science.org
April 7, 2025 at 9:43 PM
Beautiful paper
Reposted by Harrold van den Burg
How Bacteria Outsmart Plants—Then Flee the Scene!
#MicroSky #PlantScience #Pseudomonas
Our new research in Nature Microbiology uncovers the sophisticated teamwork of Pseudomonas syringae, a notorious plant pathogen.
🔗 rdcu.be/egczU
#MicroSky #PlantScience #Pseudomonas
Our new research in Nature Microbiology uncovers the sophisticated teamwork of Pseudomonas syringae, a notorious plant pathogen.
🔗 rdcu.be/egczU
Pseudomonas syringae subpopulations cooperate by coordinating flagellar and type III secretion spatiotemporal dynamics to facilitate plant infection
Nature Microbiology - Single-cell gene expression analysis reveals phenotypic heterogeneity to enable bacterial specialization over the course of plant colonization.
rdcu.be
April 3, 2025 at 2:52 PM
How Bacteria Outsmart Plants—Then Flee the Scene!
#MicroSky #PlantScience #Pseudomonas
Our new research in Nature Microbiology uncovers the sophisticated teamwork of Pseudomonas syringae, a notorious plant pathogen.
🔗 rdcu.be/egczU
#MicroSky #PlantScience #Pseudomonas
Our new research in Nature Microbiology uncovers the sophisticated teamwork of Pseudomonas syringae, a notorious plant pathogen.
🔗 rdcu.be/egczU
Reposted by Harrold van den Burg
Delighted to have our Perturb-multiome paper, led by #JorgeMartinRufino and @hemagene.bsky.social from our lab, published today in @science.org. Please check out this powerful approach to decipher gene regulatory networks enriched for genetic variation: www.science.org/doi/10.1126/...
Transcription factor networks disproportionately enrich for heritability of blood cell phenotypes
Most phenotype-associated genetic variants map to noncoding regulatory regions of the human genome, but their mechanisms remain elusive in most cases. We developed a highly efficient strategy, Perturb...
www.science.org
April 3, 2025 at 11:57 PM
Delighted to have our Perturb-multiome paper, led by #JorgeMartinRufino and @hemagene.bsky.social from our lab, published today in @science.org. Please check out this powerful approach to decipher gene regulatory networks enriched for genetic variation: www.science.org/doi/10.1126/...
Reposted by Harrold van den Burg
David Liu receives Breakthrough Prize in Life Sciences.
Liu is honored for the development of base editing and prime editing, two gene editing technologies transforming medicine. www.broadinstitute.org/news/david-l...
Liu is honored for the development of base editing and prime editing, two gene editing technologies transforming medicine. www.broadinstitute.org/news/david-l...
David Liu receives Breakthrough Prize in Life Sciences
Liu is honored for the development of base editing and prime editing, two gene editing technologies transforming medicine.
www.broadinstitute.org
April 5, 2025 at 10:43 PM
David Liu receives Breakthrough Prize in Life Sciences.
Liu is honored for the development of base editing and prime editing, two gene editing technologies transforming medicine. www.broadinstitute.org/news/david-l...
Liu is honored for the development of base editing and prime editing, two gene editing technologies transforming medicine. www.broadinstitute.org/news/david-l...
Reposted by Harrold van den Burg
Le Naour--Vernet et al. Structure‐guided insights into the biology of fungal effectors
nph.onlinelibrary.wiley.com/doi/full/10.... featuring MycFOLD effectors
nph.onlinelibrary.wiley.com/doi/full/10.... featuring MycFOLD effectors
March 28, 2025 at 8:45 PM
Le Naour--Vernet et al. Structure‐guided insights into the biology of fungal effectors
nph.onlinelibrary.wiley.com/doi/full/10.... featuring MycFOLD effectors
nph.onlinelibrary.wiley.com/doi/full/10.... featuring MycFOLD effectors
Reposted by Harrold van den Burg
Oh yes. This is new. Non-NLR and NLR pair for immune sensor and executor!
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
A wheat tandem kinase and NLR pair confers resistance to multiple fungal pathogens
Tandem kinase proteins underlie the innate immune systems of cereal plants, but how they initiate plant immune responses remains unclear. This report identifies wheat protein wheat tandem NBD 1 (WTN1)...
www.science.org
March 28, 2025 at 1:07 AM
Oh yes. This is new. Non-NLR and NLR pair for immune sensor and executor!
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Harrold van den Burg
Yes, that's a proteasome! Guess where it is....in the apoplast! Nice to see Richard Vierstra's work out! #PPStasis #proteostasis #proteasomes #PlantSci
www.nature.com/articles/s41...
www.nature.com/articles/s41...
February 18, 2025 at 4:23 PM
Yes, that's a proteasome! Guess where it is....in the apoplast! Nice to see Richard Vierstra's work out! #PPStasis #proteostasis #proteasomes #PlantSci
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Harrold van den Burg
Check out our new study! We show that Arabidopsis uses the EDS1-SAG101-NRG1 node to trigger rapid yet contained cell death. Key finding: NRG1C variant regulates this process by competing with functional NRG1s. Amazing collaboration between Jijie Chai and Parker Labs.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
February 13, 2025 at 11:09 AM
Check out our new study! We show that Arabidopsis uses the EDS1-SAG101-NRG1 node to trigger rapid yet contained cell death. Key finding: NRG1C variant regulates this process by competing with functional NRG1s. Amazing collaboration between Jijie Chai and Parker Labs.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Great new study from our lab revealing insights in Xanthomonas evolution and how loss of the Crispr genome defense was a key step for X. campestris pv campestris.
➡️ New insights into the evolution of the vascular pathogen Xanthomonas campestris involving the genome defense system CRISPR-Cas! www.cell.com/current-biol... Our data wizard 🪄@mpaauw.bsky.social sequenced almost 100 genomes and this is what he found … /
Evolution of a vascular plant pathogen is associated with the loss of CRISPR-Cas and an increase in genome plasticity and virulence genes
Paauw et al. report on the evolution of a plant pathogenic Xanthomonas pathovar and
show that the gain of mobile genetic elements and virulence factors coincided with
the loss of the CRISPR-Cas genome...
www.cell.com
February 10, 2025 at 5:15 PM
Great new study from our lab revealing insights in Xanthomonas evolution and how loss of the Crispr genome defense was a key step for X. campestris pv campestris.
Reposted by Harrold van den Burg
Lighten up your life! We built a “darkbox” with a CCD camera to image bacterial infections across entire trays of Arabidopsis plants. Our technical advance with Python image analysis pipeline out now! @havandenburg.bsky.social @nwtaks.bsky.social bsppjournals.onlinelibrary.wiley.com/doi/10.1111/...
Non‐Invasive, Bioluminescence‐Based Visualisation and Quantification of Bacterial Infections in Arabidopsis Over Time
Development of a non-invasive high-throughput phenotyping pipeline to visualise and quantify progression of bioluminescent bacterial pathogens in Arabidopsis rosettes over time.
bsppjournals.onlinelibrary.wiley.com
February 3, 2025 at 9:41 AM
Lighten up your life! We built a “darkbox” with a CCD camera to image bacterial infections across entire trays of Arabidopsis plants. Our technical advance with Python image analysis pipeline out now! @havandenburg.bsky.social @nwtaks.bsky.social bsppjournals.onlinelibrary.wiley.com/doi/10.1111/...