Julian Grünewald
@grunewald.bsky.social
Assistant Professor at TUM. CRISPR tech dev, cardiovascular gene & cell therapies. https://www.cos.tum.de/en/cos/research/gruenewald-lab/
Pinned
Julian Grünewald
@grunewald.bsky.social
· Nov 26
Very excited about the first preprint from our lab at TUM! This is a fun gene & cell therapy collaboration led by
@schmidts.bsky.social's lab. We use generative #AI
to design new #CARTcell therapies. Check out Andrea's thread & thanks to the whole team! www.biorxiv.org/content/10.1...
@schmidts.bsky.social's lab. We use generative #AI
to design new #CARTcell therapies. Check out Andrea's thread & thanks to the whole team! www.biorxiv.org/content/10.1...
Reposted by Julian Grünewald
Publication alert: Our paper on domain insertion predictions in proteins is now out in @natmethods.nature.com in its final form: rdcu.be/ey7w3
Also check out the nice perspective by @noahholzleitner.bsky.social and @grunewald.bsky.social : www.nature.com/articles/s41...
Also check out the nice perspective by @noahholzleitner.bsky.social and @grunewald.bsky.social : www.nature.com/articles/s41...
Rational engineering of allosteric protein switches by in silico prediction of domain insertion sites
Nature Methods - ProDomino is a machine leaning-based method, trained on a semisynthetic domain insertion dataset, to guide the engineering of protein domain recombination.
rdcu.be
August 4, 2025 at 12:28 PM
Publication alert: Our paper on domain insertion predictions in proteins is now out in @natmethods.nature.com in its final form: rdcu.be/ey7w3
Also check out the nice perspective by @noahholzleitner.bsky.social and @grunewald.bsky.social : www.nature.com/articles/s41...
Also check out the nice perspective by @noahholzleitner.bsky.social and @grunewald.bsky.social : www.nature.com/articles/s41...
Reposted by Julian Grünewald
Our work on rationally engineering allosteric protein switches is now out in Nature Methods: www.nature.com/articles/s41... Thanks a lot to @grunewald.bsky.social and @noahholzleitner.bsky.social for the comprehensive news and views: www.nature.com/articles/s41...
Rational engineering of allosteric protein switches by in silico prediction of domain insertion sites - Nature Methods
ProDomino is a machine leaning-based method, trained on a semisynthetic domain insertion dataset, to guide the engineering of protein domain recombination.
www.nature.com
August 4, 2025 at 11:41 AM
Our work on rationally engineering allosteric protein switches is now out in Nature Methods: www.nature.com/articles/s41... Thanks a lot to @grunewald.bsky.social and @noahholzleitner.bsky.social for the comprehensive news and views: www.nature.com/articles/s41...
Reposted by Julian Grünewald
Dual SORT LNPs for multi-organ base editing go.nature.com/3HC9EHt
Dual SORT LNPs for multi-organ base editing - Nature Biotechnology
Optimized SORT LNPs enable precise and long-lasting base editing in dual organs in a disease mouse model.
go.nature.com
June 2, 2025 at 2:48 PM
Dual SORT LNPs for multi-organ base editing go.nature.com/3HC9EHt
Reposted by Julian Grünewald
For the first time, doctors have created a customized treatment using the revolutionary gene-editing technique known as CRISPR to treat a baby with a rare, life-threatening genetic disorder.
A promising genetic treatment tailor-made for a baby born with a rare disorder
For the first time, doctors have created a customized treatment using the revolutionary gene-editing technique known as CRISPR to treat a baby with a rare, life-threatening genetic disorder.
www.npr.org
May 15, 2025 at 5:19 PM
For the first time, doctors have created a customized treatment using the revolutionary gene-editing technique known as CRISPR to treat a baby with a rare, life-threatening genetic disorder.
Reposted by Julian Grünewald
Today "a milestone in the evolution of personalized therapies for rare & ultra-rare inborn errors of metabolism"
—the 1st human to undergo custom genome editing
—from decades of NIH funded research
www.nejm.org/doi/full/10....
@nejm.org
www.nejm.org/doi/full/10....
www.nytimes.com/2025/05/15/h...
—the 1st human to undergo custom genome editing
—from decades of NIH funded research
www.nejm.org/doi/full/10....
@nejm.org
www.nejm.org/doi/full/10....
www.nytimes.com/2025/05/15/h...
May 15, 2025 at 6:05 PM
Today "a milestone in the evolution of personalized therapies for rare & ultra-rare inborn errors of metabolism"
—the 1st human to undergo custom genome editing
—from decades of NIH funded research
www.nejm.org/doi/full/10....
@nejm.org
www.nejm.org/doi/full/10....
www.nytimes.com/2025/05/15/h...
—the 1st human to undergo custom genome editing
—from decades of NIH funded research
www.nejm.org/doi/full/10....
@nejm.org
www.nejm.org/doi/full/10....
www.nytimes.com/2025/05/15/h...
Reposted by Julian Grünewald
Evolution-guided protein design of IscB for persistent epigenome editing in vivo - @zhangf.bsky.social @broadinstitute.org @hhmi.org go.nature.com/44XVBph
Evolution-guided protein design of IscB for persistent epigenome editing in vivo - Nature Biotechnology
A combination of engineering approaches is used to improve the function of IscB for a variety of editing activities in vivo.
go.nature.com
May 7, 2025 at 1:47 PM
Evolution-guided protein design of IscB for persistent epigenome editing in vivo - @zhangf.bsky.social @broadinstitute.org @hhmi.org go.nature.com/44XVBph
Reposted by Julian Grünewald
By combining high-throughput protein engineering with machine learning, bespoke SpCas9 enzymes are identified that outperform evolution-based and engineered nucleases and base editors in human cells. www.nature.com/articles/s41... #NBTHighlight
Custom CRISPR—Cas9 PAM variants via scalable engineering and machine learning - Nature
Nature - Custom CRISPR—Cas9 PAM variants via scalable engineering and machine learning
www.nature.com
April 23, 2025 at 10:57 AM
By combining high-throughput protein engineering with machine learning, bespoke SpCas9 enzymes are identified that outperform evolution-based and engineered nucleases and base editors in human cells. www.nature.com/articles/s41... #NBTHighlight
Reposted by Julian Grünewald
AlphaFold is amazing but gives you static structures 🧊
In a fantastic teamwork, @mcagiada.bsky.social and @emilthomasen.bsky.social developed AF2χ to generate conformational ensembles representing side-chain dynamics using AF2 💃
Code: github.com/KULL-Centre/...
Colab: github.com/matteo-cagia...
In a fantastic teamwork, @mcagiada.bsky.social and @emilthomasen.bsky.social developed AF2χ to generate conformational ensembles representing side-chain dynamics using AF2 💃
Code: github.com/KULL-Centre/...
Colab: github.com/matteo-cagia...
April 17, 2025 at 7:11 PM
AlphaFold is amazing but gives you static structures 🧊
In a fantastic teamwork, @mcagiada.bsky.social and @emilthomasen.bsky.social developed AF2χ to generate conformational ensembles representing side-chain dynamics using AF2 💃
Code: github.com/KULL-Centre/...
Colab: github.com/matteo-cagia...
In a fantastic teamwork, @mcagiada.bsky.social and @emilthomasen.bsky.social developed AF2χ to generate conformational ensembles representing side-chain dynamics using AF2 💃
Code: github.com/KULL-Centre/...
Colab: github.com/matteo-cagia...
Reposted by Julian Grünewald
We are super excited to share our work on #InnateImmune sensing of #AAV 🦠🧬 in the 🧠, just out in @natcomms.nature.com ! 🤩
Huge congratulations to all the team and collaborators 💪🏻
And our funders 💰
@erc.europa.eu #FondazioneTelethon and #FNIH 🙏🏻
www.nature.com/articles/s41...
Huge congratulations to all the team and collaborators 💪🏻
And our funders 💰
@erc.europa.eu #FondazioneTelethon and #FNIH 🙏🏻
www.nature.com/articles/s41...
AAV vectors trigger DNA damage response-dependent pro-inflammatory signalling in human iPSC-derived CNS models and mouse brain
Nature Communications - Costa-Verdera et. al describe a mechanism by which AAV vector genomes activate P53-mediated signalling in CNS cells involving downstream STING activation and...
www.nature.com
April 18, 2025 at 12:18 PM
We are super excited to share our work on #InnateImmune sensing of #AAV 🦠🧬 in the 🧠, just out in @natcomms.nature.com ! 🤩
Huge congratulations to all the team and collaborators 💪🏻
And our funders 💰
@erc.europa.eu #FondazioneTelethon and #FNIH 🙏🏻
www.nature.com/articles/s41...
Huge congratulations to all the team and collaborators 💪🏻
And our funders 💰
@erc.europa.eu #FondazioneTelethon and #FNIH 🙏🏻
www.nature.com/articles/s41...
Reposted by Julian Grünewald
Our latest work now online in Cell:
Rewriting regulatory DNA to dissect and reprogram gene expression
Our new method (Variant-EFFECTS) uses high-throughput prime editing + flow sorting + sequencing to precisely measure effects of noncoding variants on gene expression
Thread 👇
Rewriting regulatory DNA to dissect and reprogram gene expression
Our new method (Variant-EFFECTS) uses high-throughput prime editing + flow sorting + sequencing to precisely measure effects of noncoding variants on gene expression
Thread 👇
April 17, 2025 at 6:26 PM
Our latest work now online in Cell:
Rewriting regulatory DNA to dissect and reprogram gene expression
Our new method (Variant-EFFECTS) uses high-throughput prime editing + flow sorting + sequencing to precisely measure effects of noncoding variants on gene expression
Thread 👇
Rewriting regulatory DNA to dissect and reprogram gene expression
Our new method (Variant-EFFECTS) uses high-throughput prime editing + flow sorting + sequencing to precisely measure effects of noncoding variants on gene expression
Thread 👇
Reposted by Julian Grünewald
RFDiffusion2: a diffusion model that scaffolds enzyme active sites at the atomic level enables the generation of functional de novo enzymes
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
April 11, 2025 at 7:54 PM
RFDiffusion2: a diffusion model that scaffolds enzyme active sites at the atomic level enables the generation of functional de novo enzymes
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Julian Grünewald
www.cell.com/cell/fulltex...
ENVLPE, our system for VLP-based delivery of gene editors, is now published in @cellcellpress.bsky.social. We showed efficient delivery of base- and prime-editors in primary T cells & in vivo retinal models. All core constructs are available from @addgene.bsky.social.
ENVLPE, our system for VLP-based delivery of gene editors, is now published in @cellcellpress.bsky.social. We showed efficient delivery of base- and prime-editors in primary T cells & in vivo retinal models. All core constructs are available from @addgene.bsky.social.
Engineered nucleocytosolic vehicles for loading of programmable editors
Engineered nucleocytosolic vehicles for loading of programmable editors (ENVLPE) with
enhanced CRISPR ribonucleoprotein (RNP) loading and stabilization enable the efficient
delivery of gene-editing ef...
www.cell.com
April 9, 2025 at 11:52 PM
www.cell.com/cell/fulltex...
ENVLPE, our system for VLP-based delivery of gene editors, is now published in @cellcellpress.bsky.social. We showed efficient delivery of base- and prime-editors in primary T cells & in vivo retinal models. All core constructs are available from @addgene.bsky.social.
ENVLPE, our system for VLP-based delivery of gene editors, is now published in @cellcellpress.bsky.social. We showed efficient delivery of base- and prime-editors in primary T cells & in vivo retinal models. All core constructs are available from @addgene.bsky.social.
Reposted by Julian Grünewald
Dive into our lab’s first peer-reviewed publication—co-led with our friends @benadler.bsky.social and Muntathar Al-Shimary from the @doudna lab! If you’ve already read the preprint, don’t miss the new updates: doi.org/10.1038/s415... @naturemicrobiol.bsky.social
CRISPRi-ART enables functional genomics of diverse bacteriophages using RNA-binding dCas13d - Nature Microbiology
Leveraging RNA-targeting dCas13d enables selective interference with phage protein translation and facilitates measurement of phage gene fitness at a transcriptome-wide scale.
doi.org
February 26, 2025 at 7:55 PM
Dive into our lab’s first peer-reviewed publication—co-led with our friends @benadler.bsky.social and Muntathar Al-Shimary from the @doudna lab! If you’ve already read the preprint, don’t miss the new updates: doi.org/10.1038/s415... @naturemicrobiol.bsky.social
Reposted by Julian Grünewald
Making intrabodies from antibodies just got easier! Learn how we made 𝟭𝟵 intrabodies to bind and light up peptides and histone modifications in live cells. And thanks to Academia, all sequences are freely available. (video credit: Yuko Sato @YukoSatoT2) (1/15)
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
February 12, 2025 at 6:28 AM
Making intrabodies from antibodies just got easier! Learn how we made 𝟭𝟵 intrabodies to bind and light up peptides and histone modifications in live cells. And thanks to Academia, all sequences are freely available. (video credit: Yuko Sato @YukoSatoT2) (1/15)
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Julian Grünewald
Twitter:
LNP/mRNA therapeutics and AAV-based approaches require cell-level precision to achieve success without significant toxicity to other cells. To enable this, we developed SCP-Nano: an AI and tissue-clearing-based technology for precision nanotechnology. www.nature.com/articles/s41...
LNP/mRNA therapeutics and AAV-based approaches require cell-level precision to achieve success without significant toxicity to other cells. To enable this, we developed SCP-Nano: an AI and tissue-clearing-based technology for precision nanotechnology. www.nature.com/articles/s41...
January 14, 2025 at 3:13 PM
Twitter:
LNP/mRNA therapeutics and AAV-based approaches require cell-level precision to achieve success without significant toxicity to other cells. To enable this, we developed SCP-Nano: an AI and tissue-clearing-based technology for precision nanotechnology. www.nature.com/articles/s41...
LNP/mRNA therapeutics and AAV-based approaches require cell-level precision to achieve success without significant toxicity to other cells. To enable this, we developed SCP-Nano: an AI and tissue-clearing-based technology for precision nanotechnology. www.nature.com/articles/s41...
Reposted by Julian Grünewald
New paper from our lab (@erturklab.bsky.social)
We show how tissue clearing, light sheet microscopy, and AI-based image analysis can be combined to determine the biodistribution and drug delivery efficiency of nanocarriers throughout entire mouse bodies with single-cell resolution.
We show how tissue clearing, light sheet microscopy, and AI-based image analysis can be combined to determine the biodistribution and drug delivery efficiency of nanocarriers throughout entire mouse bodies with single-cell resolution.
Nanocarrier imaging at single-cell resolution across entire mouse bodies with deep learning - Nature Biotechnology
An integrated experimental and deep learning pipeline reveals cell-level targeting of nanocarriers in whole bodies.
www.nature.com
January 14, 2025 at 11:15 AM
New paper from our lab (@erturklab.bsky.social)
We show how tissue clearing, light sheet microscopy, and AI-based image analysis can be combined to determine the biodistribution and drug delivery efficiency of nanocarriers throughout entire mouse bodies with single-cell resolution.
We show how tissue clearing, light sheet microscopy, and AI-based image analysis can be combined to determine the biodistribution and drug delivery efficiency of nanocarriers throughout entire mouse bodies with single-cell resolution.
Reposted by Julian Grünewald
🧪1/ 🚨New paper on ecDNAs from our lab!
We reveal a strategy to engineer extrachromosomal DNA (ecDNA) amplifications in cells & mice. Let's dive in! Let’s dive in! 🧵👇
www.nature.com/articles/s41...
We reveal a strategy to engineer extrachromosomal DNA (ecDNA) amplifications in cells & mice. Let's dive in! Let’s dive in! 🧵👇
www.nature.com/articles/s41...
Engineered extrachromosomal oncogene amplifications promote tumorigenesis - Nature
Large extrachromosomal DNAs are engineered using a CRISPR- and Cre–loxP-based approach and shown to drive cancer in mouse models, with potential applications in determining the role of oncogene a...
www.nature.com
December 18, 2024 at 4:54 PM
🧪1/ 🚨New paper on ecDNAs from our lab!
We reveal a strategy to engineer extrachromosomal DNA (ecDNA) amplifications in cells & mice. Let's dive in! Let’s dive in! 🧵👇
www.nature.com/articles/s41...
We reveal a strategy to engineer extrachromosomal DNA (ecDNA) amplifications in cells & mice. Let's dive in! Let’s dive in! 🧵👇
www.nature.com/articles/s41...
Reposted by Julian Grünewald
I could not be more thrilled to announce the Nature Methods @naturemethods.bsky.social Method of the Year is Spatial Proteomics! Please see our editorial as a roadmap to the fantastic content in this special issue! www.nature.com/articles/s41...
Method of the Year 2024: spatial proteomics - Nature Methods
Approaches for profiling the spatial proteome in tissues are the basis of atlas-scale projects that are delivering on their promise for understanding biological complexity in health and disease.
www.nature.com
December 6, 2024 at 8:52 PM
I could not be more thrilled to announce the Nature Methods @naturemethods.bsky.social Method of the Year is Spatial Proteomics! Please see our editorial as a roadmap to the fantastic content in this special issue! www.nature.com/articles/s41...
Reposted by Julian Grünewald
Imagine seeing a disease's impact on every cell in the body. Now, you can! We introduce MouseMapper, an AI tool to reveal system-wide disease perturbations. It reveals obesity-induced changes in facial nerves & body-wide inflammation 👉🧵https://biorxiv.org/content/10.1101/2024.08.18.608300v1.full.pdf
December 5, 2024 at 3:43 PM
Imagine seeing a disease's impact on every cell in the body. Now, you can! We introduce MouseMapper, an AI tool to reveal system-wide disease perturbations. It reveals obesity-induced changes in facial nerves & body-wide inflammation 👉🧵https://biorxiv.org/content/10.1101/2024.08.18.608300v1.full.pdf
Reposted by Julian Grünewald
Our new preprint is out - please share!
We asked: How can we make CRISPR knockouts in vivo more efficient? And set up an assay that enables detection of CRISPR cutting with 100s-1000s of sgRNAs over entire chromosome arms in living animals.
Grab a ☕ and let’s dive in. 🧵🧪 1/n
#CRISPR #geneediting
We asked: How can we make CRISPR knockouts in vivo more efficient? And set up an assay that enables detection of CRISPR cutting with 100s-1000s of sgRNAs over entire chromosome arms in living animals.
Grab a ☕ and let’s dive in. 🧵🧪 1/n
#CRISPR #geneediting
December 2, 2024 at 3:16 PM
Our new preprint is out - please share!
We asked: How can we make CRISPR knockouts in vivo more efficient? And set up an assay that enables detection of CRISPR cutting with 100s-1000s of sgRNAs over entire chromosome arms in living animals.
Grab a ☕ and let’s dive in. 🧵🧪 1/n
#CRISPR #geneediting
We asked: How can we make CRISPR knockouts in vivo more efficient? And set up an assay that enables detection of CRISPR cutting with 100s-1000s of sgRNAs over entire chromosome arms in living animals.
Grab a ☕ and let’s dive in. 🧵🧪 1/n
#CRISPR #geneediting
Reposted by Julian Grünewald
By definition, enhancers can activate from a distance. But with increased distance between enhancer and promoter, the activation drops. To study this systematically, we build a synthetic locus: www.cell.com/molecular-ce... 1/12
Redirecting
doi.org
December 2, 2024 at 4:44 PM
By definition, enhancers can activate from a distance. But with increased distance between enhancer and promoter, the activation drops. To study this systematically, we build a synthetic locus: www.cell.com/molecular-ce... 1/12
Reposted by Julian Grünewald
2X #DilatedCardiomyopathy GWAS MATG #NatGenet 2024 reveal novel genes/cellular players
PROX1 - #LymphaticEndothelialCell
ERG - #EndothelialCell
DGKG - #SmoothMuscleCell
MAPT/Tau validated in both studies
>9k case
www.nature.com/articles/s41...
>14k case
www.nature.com/articles/s41...
PROX1 - #LymphaticEndothelialCell
ERG - #EndothelialCell
DGKG - #SmoothMuscleCell
MAPT/Tau validated in both studies
>9k case
www.nature.com/articles/s41...
>14k case
www.nature.com/articles/s41...
November 30, 2024 at 1:22 PM
2X #DilatedCardiomyopathy GWAS MATG #NatGenet 2024 reveal novel genes/cellular players
PROX1 - #LymphaticEndothelialCell
ERG - #EndothelialCell
DGKG - #SmoothMuscleCell
MAPT/Tau validated in both studies
>9k case
www.nature.com/articles/s41...
>14k case
www.nature.com/articles/s41...
PROX1 - #LymphaticEndothelialCell
ERG - #EndothelialCell
DGKG - #SmoothMuscleCell
MAPT/Tau validated in both studies
>9k case
www.nature.com/articles/s41...
>14k case
www.nature.com/articles/s41...
Reposted by Julian Grünewald
Online @cellpress.bsky.social, we reveal a protein shell that helps diatoms fix 20% of global #CO2 🧪🌊🌾🧶🧬🌍
Shimakawa et al:
www.cell.com/cell/fulltex...
Nam et al:
www.cell.com/cell/fulltex...
@biozentrum.bsky.social press release:
www.biozentrum.unibas.ch/news/detail/...
Read on for more...
1/🧵
Shimakawa et al:
www.cell.com/cell/fulltex...
Nam et al:
www.cell.com/cell/fulltex...
@biozentrum.bsky.social press release:
www.biozentrum.unibas.ch/news/detail/...
Read on for more...
1/🧵
November 29, 2024 at 3:17 PM
Online @cellpress.bsky.social, we reveal a protein shell that helps diatoms fix 20% of global #CO2 🧪🌊🌾🧶🧬🌍
Shimakawa et al:
www.cell.com/cell/fulltex...
Nam et al:
www.cell.com/cell/fulltex...
@biozentrum.bsky.social press release:
www.biozentrum.unibas.ch/news/detail/...
Read on for more...
1/🧵
Shimakawa et al:
www.cell.com/cell/fulltex...
Nam et al:
www.cell.com/cell/fulltex...
@biozentrum.bsky.social press release:
www.biozentrum.unibas.ch/news/detail/...
Read on for more...
1/🧵