🚀 Our new paper is out @natmethods.nature.com!

Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.

🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/

Field notes from a weekend PI at the #microscope: vesicle cargo lanes lighting up in real time like @FedEx. This is the ML1N PX domain in a fibroblast. Hot damn, there’s mind-blowing action under the surface of cells: this video shows 2 min of time 🤯 #Cellbio #MicroscopyMonday #SciArt #SciComm

Excited to share (and participate in) the 2nd #CedarsSinai & #Caltech Joint Symposium on Computational & Experimental Neuroscience! 🧠

Agenda includes talks on #CircuitNeuroscience #BMI #ComputationalNeuroscience #BrainInjury #Repair across institutions; registration code below

Cholinergic neuronal activity promotes diffuse midline glioma growth through muscarinic signaling: Cell www.cell.com/cell/fulltex...

I’ll say it since Daniel Stabley won’t: his NeuroImaging Lab didn’t just ride the wave they built it While others were still decoding the manual, they had the Developmental Neurobiology Dept MOSIACing like pros. Ain’t no edge but the cutting edge #MicroscopyMonday www.biorxiv.org/content/10.1...

How does tumour heterogeneity arise? How can we predict cancer cell plasticity? In 2 new studies, we trace #glioblastoma heterogeneity to a spatial cancer cell trajectory w. multimodal cell atlassing bit.ly/4mkrWgs & predict plasticity w. snRNA/ATAC+deep learning bit.ly/3FbI6Ic 🧵

Inspiring start to Cedars Sinai’s academic retreat by Shlomo Melmed, MD, who emphasizes how our unique structure enables progress towards science-based clinical innovation.

The word GET is totally off base for NIH funding. Institutions don’t get NIH dollars—they earn them. Grants are fierce competitions where only the best ideas push science forward. My last two grants ranked in the top 1% & 2%, outcompeting 98%. Like a VC pitch—except we’re curing diseases.

🧬 We’re excited to introduce D&D-seq, a single-cell technology that maps DNA:Protein interactions through molecular footprinting. Check it out here: biorxiv.org/content/10.1... #Genomics #Epigenetics

After long-time review, #nanoSPLITS is finally published. I am deeply grateful to my colleagues who made this work possible, especially @cajunscience.bsky.social. I am also grateful to the support of our single-cell proteomics community during the peer review on Nature Communication!

The nano splits paper is out! Single cell seq and single cell proteomics on the same single (one) normal sized human cell! proteomicsnews.blogspot.com/2024/12/the-...